Literature summary for 3.4.99.B2 extracted from

Kinouchi, T.; Nishio, H.; Nishiuchi, Y.; Tsunemi, M.; Takada, K.; Hamamoto, T.; Kagawa, Y.; Fujii, N.
Isolation and characterization of mammalian D-aspartyl endopeptidase (2007), Amino Acids, 32, 79-85.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	0.1 mM, 8.3% inhibition	Oryctolagus cuniculus
Aprotinin	0.1 mM, 14% inhibition	Oryctolagus cuniculus
benzoyl-L-Arg-L-His-D-Asp-CH2Cl	0.003 mM, 50% inhibition	Oryctolagus cuniculus
diisopropyl fluorophosphate	at 0.5 mM, 15.5% inhibition	Oryctolagus cuniculus
leupeptin	0.03 mM, 8.5% inhibition	Oryctolagus cuniculus
additional information	no inhibition: N-p-tosyl-L-phenylalanine chloromethyl ketone, N-p-tosyl-L-lysine chloromethyl ketone, phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane, L-alanyl-L-alanyl-L-phenylalanyl-chloromethane, chymostatin, EDTA	Oryctolagus cuniculus
pepstatin A	0.01 mM, 5.3% inhibition	Oryctolagus cuniculus
phosphoramidon	0.01 mM, 9.9% inhibition	Oryctolagus cuniculus
SDS	0.01%	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	succinyl-D-Asp-alpha-4-methylcoumaryl-7-amide	pH 8,5, 37°C	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial inner membrane	-	Mus musculus	5743	-
mitochondrial inner membrane	-	Oryctolagus cuniculus	5743	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	3 mM, activity is increased by a factor of two	Oryctolagus cuniculus
Ca2+	3 mM, activity is increased by a factor of two	Oryctolagus cuniculus
Mg2+	3 mM, activity is increased by a factor of two	Oryctolagus cuniculus
Mn2+	3 mM, activity is increased by a factor of two	Oryctolagus cuniculus
Sr2+	3 mM, activity is increased by a factor of two	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mus musculus	the enzyme may serve as a scavenger against accumulation of racemized proteins in aging	?	-	?
additional information	Oryctolagus cuniculus	the enzyme may serve as a scavenger against accumulation of racemized proteins in aging	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus
-	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	weak activity	Oryctolagus cuniculus	-
kidney	-	Oryctolagus cuniculus	-
liver	-	Mus musculus	-
liver	-	Oryctolagus cuniculus	-
lung	moderate activity	Oryctolagus cuniculus	-
ovary	weak activity	Oryctolagus cuniculus	-
small intestine	moderate activity	Oryctolagus cuniculus	-
spleen	-	Oryctolagus cuniculus	-
stomach	weak activity	Oryctolagus cuniculus	-
testis	weak activity	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme may serve as a scavenger against accumulation of racemized proteins in aging	Mus musculus	?	-	?
additional information	the enzyme may serve as a scavenger against accumulation of racemized proteins in aging	Oryctolagus cuniculus	?	-	?
additional information	no activity with acetyl-Tyr-Val-Ala-Asp-4-methylcoumaryl-7-amide, acetyl-Asp-Glu-Val-Asp-4-methylcoumaryl-7-amide, succinyl-Gly-Pro-Leu-Gly-Pro-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide, Leu-4-methylcoumaryl-7-amide, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Leu-Leu-Glu-4-methylcoumaryl-7-amide, Ala-Ala-Phe-4-methylcoumaryl-7-amide, succinyl-Ala-[D-Ser]-4-methylcoumaryl-7-amide, succinyl-[D-Ser]-4-methylcoumaryl-7-amide	Oryctolagus cuniculus	?	-	?
succinyl-D-Asp-4-methylcoumaryl-7-amide + H2O	-	Oryctolagus cuniculus	succinyl-D-Asp + 7-amino-4-methylcoumarin	-	?
[D-Asp]AEFRH[D-Asp]SGY + H2O	the D-Abeta1-10 peptide is degraded at D-Asp7 and two peptides are produced [D-Asp]AEFRHX and SGY. X is not identified in this study. L-Abeta110 peptide could not be degraded by this enzyme	Oryctolagus cuniculus	[D-Asp]AEFRH[D-Asp] + SGY	-	?

Synonyms

Synonyms	Comment	Organism
D-aspartyl endopeptidase	-	Mus musculus
D-aspartyl endopeptidase	-	Oryctolagus cuniculus
DAEP	-	Mus musculus
DAEP	-	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Oryctolagus cuniculus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.003	-	pH 8.5, 37°C	Oryctolagus cuniculus	benzoyl-L-Arg-L-His-D-Asp-CH2Cl

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Release 2023.1 (January 2023)