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BRENDA support

Literature summary for 3.4.25.2 extracted from

  • Bochtler, M.; Ditzel, L.; Groll, M.; Huber, R.
    Crystal structure of heat shock locus V (HslV) from Escherichia coli (1997), Proc. Natl. Acad. Sci. USA, 94, 6070-6074.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the amplified fragment coding for HslV-EFHHHHHH is cloned into pET12b usind restriction sites Nde and SalI, expression in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor difussion against a reservoir containing 100 mM Hepes/NaOH at pH 7.5, 200 mM sodium acetate, 0.02% NaN3 and between 9% and 14% ethanol Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7B8
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-

Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli