Cloned (Comment) | Organism |
---|---|
26S proteasome subunits phylogenetic analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of pag1-1, pa200-1-6, and T (transfer)-DNA insertion mutants in the Arabidopsis thaliana ecotype Col-0 in a T-DNA library, expression of HA-, Myc-, or FLAG-tagged PAG1 derivatives, gene At2g27020 or UniProt ID O23715. The PAG1-FLAG protein successfully integrated into the 26 S proteasome complex without perturbing CP/RP assembly or the phenotype | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MG132 | CP inhibitor | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana Col-0 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | a number of core and regulatory particle subunits undergo one or more posttranslational modifications, including partial proteolytic cleavage, acetylation, and ubiquitylation, mass spectrometric analysis of the complex, overview | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
development of an affinity method to rapidly purify intact epitope-tagged 26S proteasomes using mutant homozygous PAG1-FLAG pag1-1 line | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O | trypsin-like activity | Arabidopsis thaliana | Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O | trypsin-like activity | Arabidopsis thaliana Col-0 | Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview | Arabidopsis thaliana Col-0 | ? | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Arabidopsis thaliana | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Arabidopsis thaliana Col-0 | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 26 S proteasome is composed of two subparticles, the 20S core protease that compartmentalizes the protease active sites and the 19S regulatory particle that recognizes and translocates appropriate substrates into the core protease lumen for breakdown. The 2.5-MDa core protease-regulatory particle complex is actually a heterogeneous set of particles assembled with paralogous pairs for most subunits, mass spectrometric analysis of the complex, overview | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
additional information | activity and subunit composition of the 26 S proteasomes in plants, detailed overview. The 26 S proteasome is composed of two subparticles, the 20 S core protease that compartmentalizes the protease active sites and the 19 S regulatory particle that recognizes and translocates appropriate substrates into the core protease lumen for breakdown. A diverse and highly dynamic population of proteasomes is assembled in plants, which may expand the target specificity and functions of intracellular proteolysis, overview | Arabidopsis thaliana |