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Literature summary for 3.4.25.1 extracted from
- Enzymatic properties of the proteasome purified from starfish oocytes and its catalytic subunits involved in oocyte maturation (2000), Comp. Biochem. Physiol. C, 125C, 215-223.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-Leu-Leu-Arg | IC50: 0.85 mM for chymotrypsin-like activity, 0.0056 mM for trypsin-like activity | Patiria pectinifera |  |
| benzyloxycarbonyl-Leu-Gly-Arg | IC50: 0.11 mM for chymotrypsin-like activity, 0.0066 mM for trypsin-like activity | Patiria pectinifera | |
| benzyloxycarbonyl-Leu-Leu | 0.1 mM, 84% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 43% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 20% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, 86% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
| Benzyloxycarbonyl-Leu-Leu-Arg | IC50: 0.056 mM for chymotrypsin-like activity, 0.0014 mM for trypsin-like activity | Patiria pectinifera | |
| benzyloxycarbonyl-Leu-Ser-Arg | IC50: 0.017 mM for chymotrypsin-like activity, 0.0017 mM for trypsin-like activity | Patiria pectinifera | |
| benzyloxycarbonyl-Leu-Thr-Arg | IC50: 0.065 mM for chymotrypsin-like activity, 0.0021 mM for trypsin-like activity | Patiria pectinifera | |
| benzyloxycarbonyl-Phe-Ser-Arg | IC50: 0.0089 mM for chymotrypsin-like activity, 0.002 mM for trypsin-like activity | Patiria pectinifera | |
| chymostatin | 0.1 mM, 93% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 94% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, no inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, 93% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
| E-64-d | 0.1 mM, 30% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, no inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
| MG115 | 0.1 mM, 99% inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, 80% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 61% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
| MG132 | 0.1 mM, 98% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 13% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 82% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
| PSI | 0.1 mM, 93% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, no inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin and benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin | - |
Patiria pectinifera | |
| 0.089 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | - |
Patiria pectinifera | |
| 0.2 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | - |
Patiria pectinifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 700000 | - |
gel filtration | Patiria pectinifera |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Patiria pectinifera | the chymotrypsin-like and trypsin-like activities, but not the peptidylglutamyl peptide hydrolyzing activity plays a key role in oocyte maturation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Patiria pectinifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Patiria pectinifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oocyte | - |
Patiria pectinifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O | 11% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? | |
| benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin + H2O | 36% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? | |
| additional information | the chymotrypsin-like and trypsin-like activities, but not the peptidylglutamyl peptide hydrolyzing activity plays a key role in oocyte maturation | Patiria pectinifera | ? | - |
? | |
| succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Patiria pectinifera | ? | - |
? | |
| tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | 65% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 20S proteasome | - |
Patiria pectinifera |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0089 | - |
IC50: 0.0089 mM for chymotrypsin-like activity, 0.002 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Phe-Ser-Arg | |
| 0.017 | - |
IC50: 0.017 mM for chymotrypsin-like activity, 0.0017 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Ser-Arg | |
| 0.056 | - |
IC50: 0.056 mM for chymotrypsin-like activity, 0.0014 mM for trypsin-like activity | Patiria pectinifera | Benzyloxycarbonyl-Leu-Leu-Arg | |
| 0.065 | - |
IC50: 0.065 mM for chymotrypsin-like activity, 0.0021 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Thr-Arg | |
| 0.11 | - |
IC50: 0.11 mM for chymotrypsin-like activity, 0.0066 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Gly-Arg | |
| 0.85 | - |
IC50: 0.85 mM for chymotrypsin-like activity, 0.0056 mM for trypsin-like activity | Patiria pectinifera | acetyl-Leu-Leu-Arg | |
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