Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-Leu-Leu-Arg | IC50: 0.85 mM for chymotrypsin-like activity, 0.0056 mM for trypsin-like activity | Patiria pectinifera | |
benzyloxycarbonyl-Leu-Gly-Arg | IC50: 0.11 mM for chymotrypsin-like activity, 0.0066 mM for trypsin-like activity | Patiria pectinifera | |
benzyloxycarbonyl-Leu-Leu | 0.1 mM, 84% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 43% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 20% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, 86% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
Benzyloxycarbonyl-Leu-Leu-Arg | IC50: 0.056 mM for chymotrypsin-like activity, 0.0014 mM for trypsin-like activity | Patiria pectinifera | |
benzyloxycarbonyl-Leu-Ser-Arg | IC50: 0.017 mM for chymotrypsin-like activity, 0.0017 mM for trypsin-like activity | Patiria pectinifera | |
benzyloxycarbonyl-Leu-Thr-Arg | IC50: 0.065 mM for chymotrypsin-like activity, 0.0021 mM for trypsin-like activity | Patiria pectinifera | |
benzyloxycarbonyl-Phe-Ser-Arg | IC50: 0.0089 mM for chymotrypsin-like activity, 0.002 mM for trypsin-like activity | Patiria pectinifera | |
chymostatin | 0.1 mM, 93% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 94% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, no inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, 93% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
E-64-d | 0.1 mM, 30% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, no inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
MG115 | 0.1 mM, 99% inhibition of hydrolysis of succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, 80% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 61% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
MG132 | 0.1 mM, 98% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, 13% inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, 82% inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera | |
PSI | 0.1 mM, 93% inhibition of hydrolysis of succinyl-Leu-leu-Val-Tyr-7-amido-4-methylcoumarin, no inhibition of hydrolysis of tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin and benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin, complete inhibition of hydrolysis of benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin | Patiria pectinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin | - |
Patiria pectinifera | |
0.089 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | - |
Patiria pectinifera | |
0.2 | - |
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin | - |
Patiria pectinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
700000 | - |
gel filtration | Patiria pectinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Patiria pectinifera | the chymotrypsin-like and trypsin-like activities, but not the peptidylglutamyl peptide hydrolyzing activity plays a key role in oocyte maturation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Patiria pectinifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Patiria pectinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | - |
Patiria pectinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O | 11% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? | |
benzyloxycarbonyl-Leu-Leu-Leu-7-amido-4-methylcoumarin + H2O | 36% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? | |
additional information | the chymotrypsin-like and trypsin-like activities, but not the peptidylglutamyl peptide hydrolyzing activity plays a key role in oocyte maturation | Patiria pectinifera | ? | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Patiria pectinifera | ? | - |
? | |
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | 65% of the activity with succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | Patiria pectinifera | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
20S proteasome | - |
Patiria pectinifera |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0089 | - |
IC50: 0.0089 mM for chymotrypsin-like activity, 0.002 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Phe-Ser-Arg | |
0.017 | - |
IC50: 0.017 mM for chymotrypsin-like activity, 0.0017 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Ser-Arg | |
0.056 | - |
IC50: 0.056 mM for chymotrypsin-like activity, 0.0014 mM for trypsin-like activity | Patiria pectinifera | Benzyloxycarbonyl-Leu-Leu-Arg | |
0.065 | - |
IC50: 0.065 mM for chymotrypsin-like activity, 0.0021 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Thr-Arg | |
0.11 | - |
IC50: 0.11 mM for chymotrypsin-like activity, 0.0066 mM for trypsin-like activity | Patiria pectinifera | benzyloxycarbonyl-Leu-Gly-Arg | |
0.85 | - |
IC50: 0.85 mM for chymotrypsin-like activity, 0.0056 mM for trypsin-like activity | Patiria pectinifera | acetyl-Leu-Leu-Arg |