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Literature summary for 3.4.25.1 extracted from

  • Fernandez Murray, P.; Biscoglio, M.J.; Passeron, S.
    Purification and characterization of Candida albicans 20S proteasome. Identification of 4 proteasomal subunits (2000), Arch. Biochem. Biophys., 375, 211-219.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
chymostatin 0.05 mg/ml, activation to 170% of the original activity, peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide Candida albicans
leupeptin 0.05 mg/ml, activation to 125% of the original activity, peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide Candida albicans
polylysine stimulates caseinolytic activity Candida albicans
SDS stimulates chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide and peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-IE-(Ot-Bu)-AL aldehyde 0.002 mg/ml, 75% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.002 mM, 8% inhibition of peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide Candida albicans
benzyloxycarbonyl-LLL aldehyde 0.001 mg/ml, 69% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide Candida albicans
chymostatin 0.05 mg/ml, 98% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.05 mg/ml, 15% inhibition of trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide Candida albicans
leupeptin 0.05 mg/ml, 25% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.05 mg/ml, 88% inhibition of trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide Candida albicans
SDS trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.228
-
-
Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-GGL-4-nitroanilide + H2O chymotrypsin-like activity Candida albicans ?
-
?
benzyloxycarbonyl-GGR-2-naphthylamide + H2O trypsin-like activity Candida albicans ?
-
?
benzyloxycarbonyl-LLE-2-naphthylamide + H2O peptidyl-glutamyl-peptide-hydrolyzing activity Candida albicans ?
-
?
casein + H2O
-
Candida albicans ?
-
?

Subunits

Subunits Comment Organism
More thre proteasomal subunits are identified as alpha6/Pre5, alpha3/Y13 and alpha5/Pup2 by internal sequencing of tryptic fragments. A fourth subunit is identified as alpha7/Prs1 by immunorecognition with a monoclonal antibody specific for C8 Candida albicans

Synonyms

Synonyms Comment Organism
20S proteasome
-
Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5 chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide and peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide Candida albicans
9
-
trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide Candida albicans