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Literature summary for 3.4.25.1 extracted from

  • Tamura, T.; Nagy, I.; Lupas, A.; Lottspeich, F.; Cejka, Z.; Schoofs, G.; Tanaka, K.; De Mot, R.; Baumeister, W.
    The first characterization of a eubacterial proteasome: The 20S complex of Rhodococcus (1995), Curr. Biol., 5, 766-774.
    View publication on PubMed
Search for more literature for 3.4.25.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 31000, x * 29000, x * 25000, x * 24000, tricine-SDS-PAGE Rhodococcus sp.
25000
-
 x * 31000, x * 29000, x * 25000, x * 24000, tricine-SDS-PAGE Rhodococcus sp.
29000
-
 x * 31000, x * 29000, x * 25000, x * 24000, tricine-SDS-PAGE Rhodococcus sp.
31000
-
 x * 31000, x * 29000, x * 25000, x * 24000, tricine-SDS-PAGE Rhodococcus sp.
690000
-
 gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
20S proteasome complex Rhodococcus sp.

Reaction

Reaction Comment Organism Reaction ID
cleavage of peptide bonds with very broad specificity chymotrypsin-like activity Rhodococcus sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus sp. ?
-
 ?
succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus sp. ?
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus sp. ?
-
 ?

Subunits

Subunits Comment Organism
More x * 31000, x * 29000, x * 25000, x * 24000, tricine-SDS-PAGE Rhodococcus sp.