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Literature summary for 3.4.25.1 extracted from

  • Haire, M.F.; Clark, J.J.; Jones, M.E.E.; Hendil, K.B.; Schwartz, L.M.; Mykles, D.L.
    The multicatalytic proteinase (proteasome) of the hawkmoth, Manduca sexta: Catalytic properties and immunological comparison with the lobster enzyme complex (1995), Arch. Biochem. Biophys., 318, 15-24.
    View publication on PubMed
Search for more literature for 3.4.25.1

Activating Compound

Activating Compound Comment Organism Structure
SDS stimulates peptidylglutamyl peptide hydrolase, branched-chain amino acid preferring and caseinolytic activities Manduca sexta

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin
-
 Manduca sexta
ATP inhibits trypsin-like, peptidylglutamyl peptide hydrolase, and branched-chain amino acid preferring activities Manduca sexta
leupeptin inhibits trypsin-like activity Manduca sexta
Mg2+ inhibits trypsin like activity Manduca sexta
N-ethylmaleimide inhibits trypsin-like and chymotrypsin-like activities Manduca sexta
SDS inhibits trypsin-like and chymotrypsin-like activities Manduca sexta

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
 hawkmoth
-

Purification (Commentary)

Purification (Comment) Organism
-
 Manduca sexta

Reaction

Reaction Comment Organism Reaction ID
cleavage of peptide bonds with very broad specificity trypsin-like, chymotrypsin-like activities, peptidylglutamyl peptide hydrolase and branched-chain amino acid preferring and caseinolytic activities Manduca sexta
a

Source Tissue

Source Tissue Comment Organism Textmining
integument
-
 Manduca sexta
-
intersegmental muscle
-
 Manduca sexta
-
larva
-
 Manduca sexta
-
ovary
-
 Manduca sexta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
-
 Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-Arg-beta-naphthylamide + H2O
-
 Manduca sexta ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide + H2O
-
 Manduca sexta ?
-
 ?
benzyloxycarbonyl-Gly-Pro-Ala-Gly-Gly-p-aminobenzoate + aminopeptidase-N + H2O
-
 Manduca sexta ?
-
 ?
benzyloxycarbonyl-Gly-Pro-Ala-Leu-Ala-p-aminobenzoate + aminopeptidase-N + H2O
-
 Manduca sexta ?
-
 ?
benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O
-
 Manduca sexta ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP stimulates the caseinolytic activity Manduca sexta