Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Human breast cancer cell line, MDA-MB-231, transfected with wild-type MMP-26 cDNA shows a calcium-dependent invasive potential when compared with controls that were transfected with an inactive form of MMP-26 (E209A) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D114A | mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis | Homo sapiens |
D114A | mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively | Homo sapiens |
D165A | very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26 | Homo sapiens |
E191A | very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26 | Homo sapiens |
E209A | inactive mutant enzyme | Homo sapiens |
K189E | calcium-independent high invasiveness is observed in the K189E mutant MDA-MB-231 cell line | Homo sapiens |
K189E | mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis | Homo sapiens |
K189E | mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively | Homo sapiens |
V184D | mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | MMP-26 has one high-affinity and one low-affinity calcium binding site. High-affinity calcium binding is restored at physiologically low calcium conditions with a calcium-dissociation constant of 63 nM without inducing secondary and tertiary structural changes. Low-affinity calcium binding is restored at relatively high calcium concentrations and shows a low-affinity calcium-dissociation constant value of 0.12 mM, which is accompanied with the recovery of enzymatic activity reversibly and tertiary structural changes, but without secondary structural rearrangements | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Synonyms | Comment | Organism |
---|---|---|
endometase/matrilysin-2 | - |
Homo sapiens |
MMP-26 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
high-affinity calcium binding protects MMP-26 against thermal denaturation | Homo sapiens |