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Literature summary for 3.4.24.B4 extracted from

  • Lu, D.Y.; Yu, W.H.; Yeh, W.L.; Tang, C.H.; Leung, Y.M.; Wong, K.L.; Chen, Y.F.; Lai, C.H.; Fu, W.M.
    Hypoxia-induced matrix metalloproteinase-13 expression in astrocytes enhances permeability of brain endothelial cells (2009), J. Cell. Physiol., 220, 163-173.
    View publication on PubMed
Search for more literature for 3.4.24.B4

Protein Variants

Protein Variants Comment Organism
additional information hypoxia-induced MMP-13 overexpression is antagonized by transfection with antisense oligodeoxynucleotides of c-Fos or c-Jun. Transfection of MMP-13-shRNA markedly antagonizes the proteolysis of ZO-1 induced by hypoxic-conditioned medium Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular hypoxia increases MMP-13 protein secretion from astrocytes Rattus norvegicus
-
-
extracellular matrix
-
 Rattus norvegicus 31012
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Rattus norvegicus
Zn2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte primary Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-13
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus MMP-13-shRNA for 24 h significantly inhibits hypoxia-stimulated MMP-13 mRNA expression in cell lysate and enzyme activity in supernatant down
Rattus norvegicus hypoxia increases MMP-13 protein and mRNA levels in primary rat astrocyte cultures. c-Fos and c-Jun are involved in hypoxia-induced up-regulation of MMP-13 expression in primary cultured astrocytes up

General Information

General Information Comment Organism
physiological function MMP-13 is involved in the degradation of extracellular matrix in many kinds of tissues. MMP-13 is an important factor leading to the disorganization of ZO-1, destruction of ZO-1 tight junction protein, and hyperpermeablility of blood-brain barrier in response to hypoxia. Hypoxia-induced MMP-13 expression in astrocytes enhances paracellular permeability of brain endothelial cells Rattus norvegicus