Activating Compound | Comment | Organism | Structure |
---|---|---|---|
amino phenylmercuric acetate | activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
activation by aminophenylmercuric acetate, expression of procollagenase 3 and a C-terminal truncated form of procollagenase 3 comprising amino acid residues 249-451 in mouse myeloma cells, secretion to the cell culture medium | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tissue matrix metalloproteinase inhibitor fragments of TIMP-1 | - |
Homo sapiens | |
tissue matrix metalloproteinase inhibitor fragments of TIMP-2 | - |
Homo sapiens | |
tissue matrix metalloproteinase inhibitor hybrid N.TIMP-1/C.TIMP-2 | complex formation is not affected by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | |
tissue matrix metalloproteinase inhibitor TIMP-1 | complex formation is promoted by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | |
tissue matrix metalloproteinase inhibitor TIMP-2 | complex formation is not affected by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | |
tissue matrix metalloproteinase inhibitor TIMP-3 | complex formation is promoted by the C-terminal domain of the enzyme, inhibition mechanism, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE | Homo sapiens |
35000 | - |
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE | Homo sapiens |
50000 | - |
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE | Homo sapiens |
60000 | - |
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autoproteolysis resulting in two N-glycosylated 29 kDa fragments and one 27 kDa fragment, processing of enzyme proform via a short-living intermediate form to the active form with Tyr85 at its N-terminus | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and truncated mutant from myeloma cell culture medium | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of enzyme and enzyme fragment with synthetic peptide substrates | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly-norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2 + H2O | - |
Homo sapiens | (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly + norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2 | - |
? | |
(7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly-Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O | - |
Homo sapiens | (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly + Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 | - |
? | |
Collagen type I + H2O | wild-type enzyme, substrate binding is mediated by the C-terminal domain, which is essential for the triple helicase activity | Homo sapiens | ? | - |
? | |
collagen type I beta1,2 chain + H2O | wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | collagen type I alpha1,2 chain | - |
? | |
collagen type II + H2O | wild-type enzyme, soluble substrate | Homo sapiens | ? | - |
? | |
collagen type IV + H2O | substrate alpha2-chain and alpha1-chain, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | ? | - |
? | |
collagen type IV + H2O | wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | ? | - |
? | |
collagen type IX + H2O | wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | ? | - |
? | |
collagen type X + H2O | wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | 48 kDa fragment | - |
? | |
collagen type XIV + H2O | alpha3-chain, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | ? | - |
? | |
fibronectin + H2O | native and recombinant substrate, whole substrate molecule and fragments, wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | fragments of MW 100 kDa, 43 kDa, 35 kDa, and 29 kDa | from intact plasma fibronectin | ? | |
large tenascin C isoform + H2O | wild-type enzyme and enzyme fragment 249-451 | Homo sapiens | ? | - |
? | |
additional information | no activity with the minor cartilage collagen type XI | Homo sapiens | ? | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? | |
protein + H2O | substrate specificity, overview | Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 35000, enzyme fragment 249-451, SDS-PAGE, x * 24000, mature enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
M10.013 | Merops-ID | Homo sapiens |
matrix metalloproteinase-13 | - |
Homo sapiens |
MMP-13 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | tissue matrix metalloproteinase inhibitors and fragments | Homo sapiens |