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Literature summary for 3.4.24.B39 extracted from
- Purification and partial characterization of a novel fibrinogenase from the venom of Deinagkistrodon acutus: inhibition of platelet aggregation (2014), Protein Expr. Purif., 99, 99-105.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme has a clinical application for the therapy of thrombosis disease | Deinagkistrodon acutus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzamidine | complete inhibition at 1 mM | Deinagkistrodon acutus |  |
| additional information | not inhibited by EDTA | Deinagkistrodon acutus | |
| phenylmethylsulfonyl fluoride | complete inhibition at 1 mM | Deinagkistrodon acutus | |
| tris-(2-carboxyethyl)phosphine hydrochloride | complete inhibition at 1 mM | Deinagkistrodon acutus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no Zn2+, Ca2+, Ni2+, Co2+ and Cu2+ are detected in the purified enzyme | Deinagkistrodon acutus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, SDS-PAGE | Deinagkistrodon acutus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine fibrinogen + H2O | Deinagkistrodon acutus | the enzyme rapidly hydrolyzes the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and does not cleave the gamma-chain | ? | - |
? | |
| additional information | Deinagkistrodon acutus | the enzyme is devoid of fibrinolytic activity and also exhibits arginine esterase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinagkistrodon acutus | A2TK72 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephadex A-50 gel filtration and CM-Sephadex C-50 gel filtration | Deinagkistrodon acutus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Deinagkistrodon acutus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine fibrinogen + H2O | the enzyme rapidly hydrolyzes the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and does not cleave the gamma-chain | Deinagkistrodon acutus | ? | - |
? | |
| additional information | the enzyme is devoid of fibrinolytic activity and also exhibits arginine esterase activity | Deinagkistrodon acutus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Deinagkistrodon acutus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-Fibrinogenase | - |
Deinagkistrodon acutus |
| DAnase | - |
Deinagkistrodon acutus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Deinagkistrodon acutus | isoelectric focusing | - |
6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme's fibrinogenolytic activity is involved in its inhibition of ADP-induced platelet aggregation | Deinagkistrodon acutus |
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