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Literature summary for 3.4.24.B30 extracted from
- An extremely heat-stable extracellular proteinase (aeropyrolysin) from the hyperthermophilic archaeon Aeropyrum pernix K1 (1997), FEBS Lett., 415, 329-334.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 1%, 7% loss of acivity. 2% 2.6fold activation | Aeropyrum pernix |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | the maximal enzymatic activity is obtained at 110°C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100°C. Below 100°C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity | Aeropyrum pernix | |
| diisopropyl fluorophosphate | 1 mM, 81% inhibition | Aeropyrum pernix | |
| EDTA | 1 mM, complete loss of activity | Aeropyrum pernix | |
| EGTA | 1 mM, 96% inhibition | Aeropyrum pernix | |
| phenylmethylsulfonyl fluoride | 1 mM, 45% inhibition | Aeropyrum pernix | |
| soybean trypsin | 1 mg/ml, 82% inhibition | Aeropyrum pernix |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aeropyrum pernix | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | the maximal enzymatic activity is obtained at 110°C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100°C. Below 100°C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity | Aeropyrum pernix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, SDS-PAGE | Aeropyrum pernix |
| 53000 | - |
gel filtration | Aeropyrum pernix |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-mercaptoethanol | 1% 2-mercaptoethanol, 31% loss of activity. 5% 2-mercaptoethanol, 56% loss of activity | Aeropyrum pernix |
| dithiothreitol | 1 mM, 8% loss of activity. 5 mM, 33% loss of activity | Aeropyrum pernix |
| guanidine-HCl | 1 M, no loss of activity. 4 M, 37% loss of activity | Aeropyrum pernix |
| SDS | 1% SDS, 7% loss of acivity. 2% SDS, 2.6fold activation | Aeropyrum pernix |
| urea | 4 M, no loss of activity | Aeropyrum pernix |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
- |
- |
| Aeropyrum pernix DSM 11879 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aeropyrum pernix |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Aeropyrum pernix | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 + H2O | - |
Aeropyrum pernix | ? | - |
? | |
| 7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 + H2O | - |
Aeropyrum pernix DSM 11879 | ? | - |
? | |
| 7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O | - |
Aeropyrum pernix | ? | - |
? | |
| 7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O | - |
Aeropyrum pernix DSM 11879 | ? | - |
? | |
| 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2 + H2O | - |
Aeropyrum pernix | ? | - |
? | |
| 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2 + H2O | - |
Aeropyrum pernix DSM 11879 | ? | - |
? | |
| azocasein + H2O | - |
Aeropyrum pernix | ? | - |
? | |
| azocasein + H2O | - |
Aeropyrum pernix DSM 11879 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
in absence of CaCl2 | Aeropyrum pernix |
| 110 | - |
in the presence of 1 mM CaCl2 | Aeropyrum pernix |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 130 | 80°C: about 50% of maximal activity, 130°C: about 35% of maximal activity, in the presence of 1 mM CaCl2 | Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 120 | - |
half-life: 2.5 h, in the presence of 1 mM CaCl2 | Aeropyrum pernix |
| 125 | - |
half-life: 1.2 h, in the presence of 1 mM CaCl2 | Aeropyrum pernix |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | azocasein hydrolysis | Aeropyrum pernix |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | pH 4.5: about 30% of maximal activity, pH 9.0: about 65% loss of maximal activity | Aeropyrum pernix |
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