Cloned (Comment) | Organism |
---|---|
expression of catalytic site comprising residues 98-272 and of mutants A235P and E216A in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A235P | mutant shows about 10fold increased activity with alpha1-proteinase inhibitor | Homo sapiens |
E216A | active site mutant, catalytically inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
matrix metalloproteinase inhibitor AG3340 | broad spectrum inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.148 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
0.207 | - |
GGYAELRMGG | pH 7.5, 37°C | Homo sapiens | |
0.294 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
0.705 | - |
GGAANLVRGG | pH 7.5, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant catalytic domain and mutants from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | reaction and inhibition mechanism | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha1-antitrypsin inhibitor + H2O | - |
Homo sapiens | ? | - |
? | |
GAAGAMFLEA + H2O | - |
Homo sapiens | GAAGA + MFLEA | - |
? | |
GGAANLVRGG + H2O | - |
Homo sapiens | GGAAN + LVRGG | - |
? | |
GGPLALWARGG + H2O | - |
Homo sapiens | GGPLA + LWARGG | - |
? | |
GGPLGLYAGG + H2O | - |
Homo sapiens | GGPLG + LYAGG | - |
? | |
GGQPRGVWGG + H2O | - |
Homo sapiens | GGQPRG + VWGG | - |
? | |
GGTDAWLSGG + H2O | - |
Homo sapiens | GGTDA + WLSGG | - |
? | |
GGYAELRMGG + H2O | best substrate | Homo sapiens | GGYAE + LRMGG | - |
? | |
additional information | wild-type enzyme does not cleave extracellular matrix proteins such as collagen, laminin, fibronectin, and elastin | Homo sapiens | ? | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? | |
protein + H2O | substrate specificity, enzyme prefers 2 consensus sequences: 1. A(A/Q)(N/A)_(L/Y)(T/V/M/R)(R/K) and 2. G(G/A)E_LR, with _ marking the cleavage sites, enzyme also cleaves peptides containing Ala in position 3, Ala in position 1, and Leu/Tyr in position 1', peptides with proline in position 3 are poor substrates | Homo sapiens | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M10.007 | Merops-ID | Homo sapiens |
matrix metalloproteinase-11 | - |
Homo sapiens |
MMP-11 proteinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.69 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
1.65 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3 | 6 | GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
3.08 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant mutant A235P | Homo sapiens | |
6.08 | - |
GGYAELRMGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens | |
6.08 | - |
GGAANLVRGG | pH 7.5, 37°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.6 | assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000006 | - |
matrix metalloproteinase inhibitor AG3340 | pH 7.6, 37°C | Homo sapiens |