Literature summary for 3.4.24.B24 extracted from

Kornd�rfer, I.P.; Kanitz, A.; Danzer, J.; Zimmer, M.; Loessner, M.J.; Skerra, A.
Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family (2008), Acta Crystallogr. Sect. D, D64, 644-650.

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Application

Application	Comment	Organism
biotechnology	bacteriophage peptidoglycan-hydrolyzing enzymes have found several applications in biotechnology and medicine, e.g. for surface-decontamination purposes, for biopreservation of food and feed and as potential topical antimicrobials	Listeria phage A500

Cloned(Commentary)

Cloned (Comment)	Organism
into the pASK-IBA5 vector for expression of truncated Ply500 fragments, EAD500(1-141), EAD500(1-151), EAD500(1-163), in Escherichia coli JM83 cells	Listeria phage A500

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of the enzymatically active domain of Ply500 is solved at 1.8 A resolution	Listeria phage A500

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	the enzymatically active domain, EAD, is linked to a cell-wall-binding domain, CBD	Listeria phage A500	5618	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Listeria phage A500

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33400	-	-	Listeria phage A500

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Listeria phage A500	peptidoglycan hydrolysis	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Listeria phage A500	-	Listeria bacteriophage	-

Purification (Commentary)

Purification (Comment)	Organism
using a streptavidin and a Superdex-75 gel-filtration column	Listeria phage A500

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	peptidoglycan hydrolysis	Listeria phage A500	?	-	?

Synonyms

Synonyms	Comment	Organism
L,D-endopeptidase	-	Listeria phage A500
L-alanoyl-D-glutamate endopeptidase	-	Listeria phage A500
L-alanoyl-D-glutamate peptidase	-	Listeria phage A500
Ply500	-	Listeria phage A500

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Release 2023.1 (January 2023)