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Literature summary for 3.4.24.B19 extracted from

  • Leonhard, K.; Stiegler, A.; Neupert, W.; Langer, T.
    Chaperone-like activity of the AAA domain of the yeast Yme1 AAA protease (1999), Nature, 398, 348-351.
    View publication on PubMed
Search for more literature for 3.4.24.B19

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminal residues 250-313 of the AAA domain as GST-fusion protein in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
E541Q point mutation, inactive Saccharomyces cerevisiae
K327R point mutation at the ATP-binding site, catalytically inactive Saccharomyces cerevisiae
additional information N-terminal AAA-domain fragment, expressed in Escherichia coli, is sufficient for substrate binding, but the efficiency is reduced by 5fold compared to the wild-type enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
methotrexate inhibits binding of the protein substrate to the enzyme's AAA domain Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane outer surface of Saccharomyces cerevisiae 5743
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
unassembled cytochrome oxidase 2 Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 Yme1 constitutes the i-AAA protease
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant GST-fusion AAA-domain N-terminal fragment from Escherichia coli Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
proteolytic degradation of proteins substrate recognition mechanism model Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
loosely folded Yta10(161)-dihydrofolate reductase + H2O in vitro import into mitochondria, not intact wild-type dihydrofolate reductase Saccharomyces cerevisiae ?
-
 ?
unassembled cytochrome oxidase 2
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
More cooperative interactions between subunits might increase the affinity of assembled Yme1 in vivo Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
M41.004 Merops-ID Saccharomyces cerevisiae
YME1
-
 Saccharomyces cerevisiae
YME1 AAA protease
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Saccharomyces cerevisiae
additional information no activity with beta-gamma-imidoadenosine 5'-phosphate, i.e. AMP-PNP Saccharomyces cerevisiae