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Literature summary for 3.4.24.B17 extracted from

  • Li, W.; Rao, D.K.; Kaur, P.
    Dual role of the metalloprotease FtsH in biogenesis of the DrrAB drug transporter (2013), J. Biol. Chem., 288, 11854-11864.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hms174(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
H417A/E418Q/H421A the mutant shows 106% ATPase activity compared to the wild type enzyme Escherichia coli
K198N the mutant shows 12% ATPase activity compared to the wild type enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-dependent metalloprotease Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
doxorubicin resistance protein B + H2O Escherichia coli
-
 ?
-
 ?
doxorubicin resistance protein B + H2O Escherichia coli AR796
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli AR796
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O
-
 Escherichia coli ?
-
 ?
alpha-casein + H2O
-
 Escherichia coli AR796 ?
-
 ?
ATP + H2O
-
 Escherichia coli ?
-
 ?
ATP + H2O
-
 Escherichia coli AR796 ?
-
 ?
doxorubicin resistance protein B + H2O
-
 Escherichia coli ?
-
 ?
doxorubicin resistance protein B + H2O
-
 Escherichia coli AR796 ?
-
 ?

Synonyms

Synonyms Comment Organism
FtsH
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction expression of doxorubicin resistance protein B alone or doxorubicin resistance protein AB together in FtsH-deficient cells results in growth inhibition Escherichia coli
metabolism the enzyme facilitates refolding of previously misassembled membrane protein doxorubicin resistance protein AB Escherichia coli