Cloned (Comment) | Organism |
---|---|
expression of His-Myc-tagged enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral | Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Escherichia coli | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Hi-Myc-tagged enzyme | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | degradation of membrane proteins | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused, reconstituted enzyme is accessible to proteinase K because the ATPase and protease are exposed outside the vesicles | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused | Escherichia coli | ? | - |
? | |
protein + H2O | - |
Escherichia coli | peptides | - |
? | |
protein + H2O | degradation probably involves dislocation of the substrate membrane protein to the cytosol | Escherichia coli | peptides | - |
? | |
YccA-(P3)-PhoA + H2O | recombinant fusion protein | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FtsH | - |
Escherichia coli |
M41.001 | Merops-ID | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Escherichia coli |