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Literature summary for 3.4.24.B17 extracted from

  • Akiyama, Y.; Ito, K.
    Reconstitution of membrane proteolysis by FtsH (2003), J. Biol. Chem., 278, 18146-18153.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-Myc-tagged enzyme Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Escherichia coli
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Hi-Myc-tagged enzyme Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
proteolytic degradation of proteins degradation of membrane proteins Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused, reconstituted enzyme is accessible to proteinase K because the ATPase and protease are exposed outside the vesicles Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information construction of an in vitro reaction system in which the enzyme is membrane embedded and not solubilized by detergent, two inverted membrane vesicles or proteoliposomes, one bearing the enzyme, the other bearing the substrate protein, are fused Escherichia coli ?
-
?
protein + H2O
-
Escherichia coli peptides
-
?
protein + H2O degradation probably involves dislocation of the substrate membrane protein to the cytosol Escherichia coli peptides
-
?
YccA-(P3)-PhoA + H2O recombinant fusion protein Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
FtsH
-
Escherichia coli
M41.001 Merops-ID Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Escherichia coli