Cloned (Comment) | Organism |
---|---|
PHEX gene, i.e. phosphate-regulating gene with homologies to endopeptidase on the X-chromosome, expression as soluble secreted mutant form secPHEX, lacking residues 1-45, in LLC-PK1 porcine kidney cells, deletion mutant enzyme shows catalytic properties similar to the wild-type enzyme | Homo sapiens |
General Stability | Organism |
---|---|
NaCl required for stability of the purified enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by thiorphan of wild-type and soluble enzyme form | Homo sapiens | |
NaCl | 2fold lower kcat/Km, but required for stability | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.0013 | - |
o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.0015 | - |
o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.0017 | - |
o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.0024 | - |
o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.003 | - |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH | pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.003 | - |
o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.004 | - |
o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.005 | - |
o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.007 | - |
o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.009 | - |
o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.013 | - |
o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine | pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.013 | - |
o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.015 | - |
o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.017 | - |
o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.022 | - |
o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.032 | - |
o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.037 | - |
o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.047 | - |
o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.053 | - |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 | pseudo-first-order conditions, recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme form from porcine kidney cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | preference for an acidic residue at position P'1, especially for aspartic acid, glutamic acid gives 5fold lower activity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity of wild-type and soluble enzyme form with o-aminobenzoyl-RL-N-(2,4-dinitrophenyl)ethylene diamine, no activity with o-aminobenzoyl-GFSEYK-(2,4-dinitrophenyl)-NH2 | Homo sapiens | ? | - |
? | |
o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Leu-Asp bond | Homo sapiens | o-aminobenzoyl-A110WL + DSGVQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Ser-Asp bond | Homo sapiens | o-aminobenzoyl-D124HLS + DTSTQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-G197QR + DSQAQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-G337SN + DIMGQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-G386SS + DAAEQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 + H2O | cleavage site is the Ser-Asp bond | Homo sapiens | o-aminobenzoyl-GFS + DYK-(2,4-dinitrophenyl)-NH2 | - |
? | |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH + H2O | best substrate, cleavage site is the Ser-Asp bond | Homo sapiens | o-aminobenzoyl-GFS + DYK-(2,4-dinitrophenyl)-OH | - |
? | |
o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | cleavage site is the Ser-Asp bond | Homo sapiens | o-aminobenzoyl-GFS + DYQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-GFSEY-(2,4-dinitrophenyl)K + H2O | low activity, cleavage site is the Ser-Asp bond | Homo sapiens | o-aminobenzoyl-GFS + EY-(2,4-dinitrophenyl)K | - |
? | |
o-aminobenzoyl-IPSDFEGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-IPS + DFEGQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on residues 107-139 sequence of the enzyme, cleavage site is the Leu-Asp bond | Homo sapiens | o-aminobenzoyl-L134EL + DSRQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Met-Asp bond | Homo sapiens | o-aminobenzoyl-L94MM + DFRGQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Tyr-Asp bond | Homo sapiens | o-aminobenzoyl-N122GY + DVYHQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-N449EM + DSFNQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Asp-Asp bond | Homo sapiens | o-aminobenzoyl-R175RHTQSAED + DSERQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Asp-Asp bond | Homo sapiens | o-aminobenzoyl-R175RHTRSAED + DSERQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-R441GL + DNEIQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-R506RD + DSSEQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Glu-Asp bond | Homo sapiens | o-aminobenzoyl-R76SE + DAGFQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the FGF-23 protein sequence, cleavage site is the Glu-Asp bond | Homo sapiens | o-aminobenzoyl-S212AE + DNSPQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-S513S + DSGSQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O | sequence is based on the MEPE protein sequence | Homo sapiens | o-aminobenzoyl-T294HL + DTKKQ-N-(2,4-dinitrophenyl)ethylene diamine | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M13.091 | Merops-ID | Homo sapiens |
PHEX | encoded by PHEx i.e. phosphate-regulating gene with homologies to endopeptidase on the X-chromosome | Homo sapiens |
PHEX | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
o-aminobenzoyl-N122GYDVYHQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.01 | - |
o-aminobenzoyl-G337SNDIMGQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.02 | - |
o-aminobenzoyl-T294HLDTKKQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.03 | - |
o-aminobenzoyl-R441GLDNEIQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.04 | - |
o-aminobenzoyl-A110WLDSGVQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.05 | - |
o-aminobenzoyl-L134ELDSRQ-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.06 | - |
o-aminobenzoyl-R76SEDAGFQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.1 | - |
o-aminobenzoyl-D124HLSDTSTQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.15 | - |
o-aminobenzoyl-R175RHTQSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.2 | - |
o-aminobenzoyl-R175RHTRSAEDDSERQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.2 | - |
o-aminobenzoyl-S513SDSGSQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.2 | - |
o-aminobenzoyl-G197QRDSQAQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.3 | - |
o-aminobenzoyl-G386SSDAAEQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.3 | - |
o-aminobenzoyl-S212AEDNSPQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.3 | - |
o-aminobenzoyl-N449EMDSFNQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.5 | - |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-OH | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.5 | - |
o-aminobenzoyl-R506RDDSSEQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.6 | - |
o-aminobenzoyl-GFSDYQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
0.8 | - |
o-aminobenzoyl-L94MMDFRGQ-N-(2,4-dinitrophenyl)ethylene diamine | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens | |
1.3 | - |
o-aminobenzoyl-GFSDYK-(2,4-dinitrophenyl)-NH2 | recombinant soluble enzyme, pH 5.5, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | - |
Homo sapiens |