Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of Ca2+ alone | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
PHEX gene, expression of wild-type and mutants in LLC-PK1 porcine kidney cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E581V | site-directed mutagenesis, soluble secreted mutant secPHEXE581V, inactive | Homo sapiens |
additional information | construction of an active soluble secreted mutant enzyme form secPHEX, enzyme mutations are responsible for X-chromosome linked hypophosphataemia | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 1 mM | Homo sapiens | |
diphosphate | 50% inhibition at 2.5 mM | Homo sapiens | |
EDTA | complete inhibition at 1 mM | Homo sapiens | |
additional information | no effect of Ca2+ alone | Homo sapiens | |
osteocalcin | 50% inhibition at 0.0036 mM, Ca2+ reverses the effect | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-metalloprotease | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
100000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
105000 | - |
x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Homo sapiens | enzyme mutations are responsible for X-chromosome linked hypophosphataemia | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble secreted mutant enzyme forms | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | at the N-terminus of aspartate residues, the S1' pocket can accomodate negatively charged side chains of amino acid residues | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with [Leu]enkephalin, alpha-endorphin, substance P, bradykinin, big-endothelin-1, endothelin-1, alpha-calcitonin, gene-related peptide alpha-CGRP, calcitonin, osteocalcin, parathyroid-hormone-related peptide 1-84, parathyroid-hormone-related peptide 1-34, and osteogenic growth peptide | Homo sapiens | ? | - |
? | |
parathyroid-hormone-related peptide 107-139 + H2O | - |
Homo sapiens | L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucyl-L-alpha-aspartyl-L-serylglycyl-L-valyl-L-threonylglycyl-L-serylglycyl-L-leucyl-L-alpha-glutamylglycyl-L-a-aspartyl-L-histidyl-L-leucyl-L-serine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucyl-L-alpha-aspartyl-L-seryl-L-arginine + L-threonyl-L-arginyl-L-seryl-L-alanyl-L-tryptophyl-L-leucine + L-alpha-aspartyl-L-threonyl-L-seryl-L-threonyl-L-threonyl-L-seryl-L-leucyl-L-alpha-glutamyl-L-leucine + L-alpha-aspartyl-L-seryl-L-arginine | product determination | ? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? | |
protein + H2O | enzyme mutations are responsible for X-chromosome linked hypophosphataemia | Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, recombinant soluble, deglycosylated mutant, SDS-PAGE, x * 100000, recombinant soluble mutant, SDS-PAGE, x * 105000, native wild-type, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
M13.091 | Merops-ID | Homo sapiens |
PHEX | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity rapidly decreases at more basic pH values | Homo sapiens |