Cloned (Comment) | Organism |
---|---|
DNA determination and analysis, expression of wild-type and C-terminally truncated mutant as His-tagged protein in Spodoptera frugiperda Sf9 cells via baculovirus infection | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of C-terminally truncated enzyme version | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | type II membrane-bound zinc metalloprotease | Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc metalloprotease | Homo sapiens | |
Zinc | type II membrane-bound zinc metalloprotease | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
86000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
100000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Mus musculus | - |
peptides | - |
? | |
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
type II membrane-bound zinc metalloprotease | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 6 N-glycosylation sites, recombinant and wild-type enzyme | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant from SF9 insect cells as His-tagged protein | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | enzyme is an endopeptidase | Homo sapiens | |
proteolytic degradation of proteins | enzyme is an endopeptidase | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MC3T3-E1 cell | osteoblast cell culture | Mus musculus | - |
MG-63 cell | osteoblast cell culture | Homo sapiens | - |
osteoblast | - |
Mus musculus | - |
osteoblast | cell culture | Homo sapiens | - |
SAOS-2 cell | osteoblast cell culture | Homo sapiens | - |
U2-OS cell | osteoblast cell culture | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | human stanniocalcin STC-1 is no substrate, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Mus musculus | ? | - |
? | |
additional information | human stanniocalcin STC-1, casein and wild-type as well as mutant FGF-23 proteins are no substrates, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Homo sapiens | ? | - |
? | |
protein + H2O | - |
Mus musculus | peptides | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
M13.091 | Merops-ID | Mus musculus |
M13.091 | Merops-ID | Homo sapiens |
PHEX | - |
Mus musculus |
PHEX | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Mus musculus |