Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutated forms of rNEP2 in AtT20 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
P751A | no hydrolysis of succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | Rattus norvegicus |
P764Y | kcat/Km is 2.6fold lower than wild-type enzyme | Rattus norvegicus |
W774A | kcat/Km is 1.1fold lower than wild-type enzyme | Rattus norvegicus |
W774F | kcat/Km is nearly identical to wild-type value | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neprilysin 2 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme W774A | Rattus norvegicus | |
3.5 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme P764Y | Rattus norvegicus | |
7.8 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme W774F | Rattus norvegicus | |
8.7 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus |