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Literature summary for 3.4.24.B14 extracted from

  • Ikeda, K.; Emoto, N.; Raharjo, S.B.; Nurhantari, Y.; Saiki, K.; Yokoyama, M.; Matsuo, M.
    Molecular identification and characterization of novel membrane-bound metalloprotease, the soluble secreted form of which hydrolyzes a variety of vasoactive peptides (1999), J. Biol. Chem., 274, 32469-32477.
    View publication on PubMed
Search for more literature for 3.4.24.B14

Cloned(Commentary)

Cloned (Comment) Organism
isozymes SEP and SEPDELTA, DNA determination and analysis, transient expression of both isoforms in CHO cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibition of the soluble isozyme SEP Mus musculus
additional information no inhibition by captopril Mus musculus
phosphoramidon inhibition of the soluble isozyme SEP Mus musculus
thiorphan inhibition of the soluble isozyme SEP Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular isozyme SEP also is secreted Mus musculus
-
-
membrane isozymes SEP and SEPDELTA Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metalloprotease Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus
126000
-
 x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9JLI3 2 isozymes SEP and splicing variant SEPDELTA which lacks 69 base pair nucleotide segment following the transmembrane helix
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein soluble isozyme SEP, and membrane-bound isozyme SEP and SEPDELTA Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland isozymes SEP and SEPDELTA Mus musculus
-
brain isozymes SEP and SEPDELTA Mus musculus
-
heart isozymes SEP and SEPDELTA Mus musculus
-
intestine isozymes SEP and SEPDELTA Mus musculus
-
kidney isozymes SEP and SEPDELTA Mus musculus
-
liver isozymes SEP and SEPDELTA Mus musculus
-
lung isozymes SEP and SEPDELTA Mus musculus
-
additional information in most cases isozyme SEPDELTA shows higher expression rates than isozyme SEP Mus musculus
-
ovary isozyme SEP Mus musculus
-
testis isozyme SEP, very low amount of isozyme SEPDELTA Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Mus musculus ?
-
 ?
atrial natriuretic peptide + H2O
-
 Mus musculus ?
-
 ?
bradykinin + H2O
-
 Mus musculus ?
-
 ?
endothelin-1 + H2O
-
 Mus musculus ?
-
 ?
protein + H2O
-
 Mus musculus peptides
-
 ?
protein + H2O broad substrate specificity Mus musculus peptides
-
 ?
Substance P + H2O
-
 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
M13.008 Merops-ID Mus musculus
More may be identical with neprilysin EC 3.4.24.11 Mus musculus
SEP
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 sharp pH-dependence Mus musculus