Literature summary for 3.4.24.B11 extracted from

Kuno, K.; Terashima, Y.; Matsushima, K.
ADAMTS-1 is an active metalloproteinase associated with the extracellular matrix (1999), J. Biol. Chem., 274, 18821-18826.

Search for more literature for 3.4.24.B11

Cloned(Commentary)

Cloned (Comment)	Organism
adamts-1 gene maps to chromosome 16, region C3-C5, transient expression in COS-7 cells of FLAG-tagged wild-type and deletion mutants and mutant E386Q	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
E386Q	oligonucleotide primer mutagenesis, mutation within the zinc binding motif, loss of zinc-binding capacity, loss of activity and capacity to complex with alpha2-macroglobulin	Mus musculus
additional information	construction of soluble C-terminal deletion mutants, that are still able to form complexes with alpha2-macroglobulin	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	inhibition of complex formation with alpha2-macroglobulin	Mus musculus
additional information	no inhibition by leupeptin, aprotinin, phenylmethylsulfonyl floride, and pepstatin	Mus musculus
Phenanthroline	inhibition of complex formation with alpha2-macroglobulin	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	secreted protein binds to the matrix	Mus musculus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	metalloprotease, contains zinc binding motif HEXXHXXXXXHD in the metalloprotease domain	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
proteins + H2O	Mus musculus	enzyme is involved in the inflammatory process	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	processing by furin endopeptidase in vivo	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha2-macroglobulin + H2O	cleavage of the bait region of alpha2-macroglobulin, complex formation with alpha2-macroglobulin is dependent on the proteolytic activity	Mus musculus	?	-	?
proteins + H2O	-	Mus musculus	peptides	-	?
proteins + H2O	enzyme is involved in the inflammatory process	Mus musculus	peptides	-	?

Synonyms

Synonyms	Comment	Organism
ADAMTS-1	-	Mus musculus
M12.222	Merops-ID	Mus musculus

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Release 2023.1 (January 2023)