Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in insect cells via baculovirus infection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catechin gallate esters | from green tea Camellia sinensis, strong inhibition, independent of Zn2+, reversible | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloprotease | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
x * 89000, recombinant mature enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proteins + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UHI8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | recombinant enzyme is also processed in insect cells | Homo sapiens |
Purification (Comment) | Organism |
---|---|
partially, recombinant His-tagged enzyme from insect cells, 200fold | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of proteins | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
partially purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aggrecan + H2O | several cleavage sites are determined | Homo sapiens | 11 aggrecan fragment | product determination, overview | ? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
proteins + H2O | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89000, recombinant mature enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS1 | - |
Homo sapiens |
M12.222 | Merops-ID | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |