Protein Variants | Comment | Organism |
---|---|---|
E351Q | inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.01 mM Co2+ inhibits the activity by 80%, while increasing concentrations rescue the lost activity | Homo sapiens | |
EDTA | 20 mM | Homo sapiens | |
Hg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens | |
additional information | not affected by Fe2+ | Homo sapiens | |
N-TIMP-3 | the N-terminal domain of TIMP-3 inhibits ADAM12-S | Homo sapiens | |
NaCl | - |
Homo sapiens | |
Ni2+ | low concentrations of Ni2+ inhibit ADAM12-S drastically | Homo sapiens | |
TIMP-1 | weak inhibitor | Homo sapiens | |
TIMP-2 | - |
Homo sapiens | |
Zn2+ | when concentrations exceed 0.05 mM, activity is negatively affected, and at a concentration of 0.5 mM Zn2+, substrate degradation is only just detectable | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
S-carboxymethylated transferrin | ADAM12-S, at 37°C | Homo sapiens | |
0.0165 | - |
S-carboxymethylated transferrin | ADAM12-PC, at 37°C | Homo sapiens | |
0.0192 | - |
S-carboxymethylated transferrin | ADAM12-PCD, at 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Mg2+ | marginal stimulatory effect | Homo sapiens | |
Zn2+ | addition of ZnCl2 at concentrations below 0.05 mM increases the activity of ADAM12-S by approximately 30% | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betacellulin + H2O | - |
Homo sapiens | ? | - |
? | |
dabcyl-LAQA(homo)PheRSK(5FAM)-NH2 + H2O | a 5-carboxamido-fluorescein labelled substrate | Homo sapiens | ? | - |
? | |
epidermal growth factor + H2O | - |
Homo sapiens | ? | - |
? | |
heparin binding epidermal growth factor-like growth factor + H2O | - |
Homo sapiens | ? | - |
? | |
insulin-like growth factor binding protein-3 + H2O | - |
Homo sapiens | ? | - |
? | |
insulin-like growth factor binding protein-5 + H2O | - |
Homo sapiens | ? | - |
? | |
placental leucine aminopeptidase + H2O | - |
Homo sapiens | ? | - |
? | |
S-carboxymethylated transferrin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAM-PCD | encodes the pro domain, catalytic domain, and disintegrin domain | Homo sapiens |
ADAM12 | - |
Homo sapiens |
ADAM12-PC | consists of the pro domain and catalytic domain | Homo sapiens |
ADAM12-S | a secreted splice variant | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
S-carboxymethylated transferrin | ADAM12-S, at 37°C | Homo sapiens | |
0.18 | - |
S-carboxymethylated transferrin | ADAM12-PCD, at 37°C | Homo sapiens | |
0.47 | - |
S-carboxymethylated transferrin | ADAM12-PC, at 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000032 | - |
N-TIMP-3 | ADAM12-PC | Homo sapiens | |
0.0000093 | - |
TIMP-2 | apparent value, ADAM12-PC | Homo sapiens | |
0.0000125 | - |
N-TIMP-3 | ADAM12-S | Homo sapiens | |
0.000044 | - |
TIMP-2 | apparent value, ADAM12-S | Homo sapiens | |
0.00011 | - |
TIMP-1 | apparent value, ADAM12-PC | Homo sapiens | |
0.000454 | - |
TIMP-1 | apparent value, ADAM12-S | Homo sapiens |