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Literature summary for 3.4.24.B10 extracted from

  • Jacobsen, J.; Visse, R.; Sorensen, H.P.; Enghild, J.J.; Brew, K.; Wewer, U.M.; Nagase, H.
    Catalytic properties of ADAM12 and its domain deletion mutants (2008), Biochemistry, 47, 537-547.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E351Q inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.01 mM Co2+ inhibits the activity by 80%, while increasing concentrations rescue the lost activity Homo sapiens
EDTA 20 mM Homo sapiens
Hg2+
-
Homo sapiens
Mn2+
-
Homo sapiens
additional information not affected by Fe2+ Homo sapiens
N-TIMP-3 the N-terminal domain of TIMP-3 inhibits ADAM12-S Homo sapiens
NaCl
-
Homo sapiens
Ni2+ low concentrations of Ni2+ inhibit ADAM12-S drastically Homo sapiens
TIMP-1 weak inhibitor Homo sapiens
TIMP-2
-
Homo sapiens
Zn2+ when concentrations exceed 0.05 mM, activity is negatively affected, and at a concentration of 0.5 mM Zn2+, substrate degradation is only just detectable Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
S-carboxymethylated transferrin ADAM12-S, at 37°C Homo sapiens
0.0165
-
S-carboxymethylated transferrin ADAM12-PC, at 37°C Homo sapiens
0.0192
-
S-carboxymethylated transferrin ADAM12-PCD, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Mg2+ marginal stimulatory effect Homo sapiens
Zn2+ addition of ZnCl2 at concentrations below 0.05 mM increases the activity of ADAM12-S by approximately 30% Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betacellulin + H2O
-
Homo sapiens ?
-
?
dabcyl-LAQA(homo)PheRSK(5FAM)-NH2 + H2O a 5-carboxamido-fluorescein labelled substrate Homo sapiens ?
-
?
epidermal growth factor + H2O
-
Homo sapiens ?
-
?
heparin binding epidermal growth factor-like growth factor + H2O
-
Homo sapiens ?
-
?
insulin-like growth factor binding protein-3 + H2O
-
Homo sapiens ?
-
?
insulin-like growth factor binding protein-5 + H2O
-
Homo sapiens ?
-
?
placental leucine aminopeptidase + H2O
-
Homo sapiens ?
-
?
S-carboxymethylated transferrin + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ADAM-PCD encodes the pro domain, catalytic domain, and disintegrin domain Homo sapiens
ADAM12
-
Homo sapiens
ADAM12-PC consists of the pro domain and catalytic domain Homo sapiens
ADAM12-S a secreted splice variant Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
S-carboxymethylated transferrin ADAM12-S, at 37°C Homo sapiens
0.18
-
S-carboxymethylated transferrin ADAM12-PCD, at 37°C Homo sapiens
0.47
-
S-carboxymethylated transferrin ADAM12-PC, at 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000032
-
N-TIMP-3 ADAM12-PC Homo sapiens
0.0000093
-
TIMP-2 apparent value, ADAM12-PC Homo sapiens
0.0000125
-
N-TIMP-3 ADAM12-S Homo sapiens
0.000044
-
TIMP-2 apparent value, ADAM12-S Homo sapiens
0.00011
-
TIMP-1 apparent value, ADAM12-PC Homo sapiens
0.000454
-
TIMP-1 apparent value, ADAM12-S Homo sapiens