Literature summary for 3.4.24.89 extracted from

Schacherl, M.; Pichlo, C.; Neundorf, I.; Baumann, U.
Structural basis of proline-proline peptide bond specificity of the metalloprotease Zmp1 implicated in motility of Clostridium difficile (2015), Structure, 23, 1632-1642.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of Zmp1 in its unbound and peptide-bound forms. The structure analysis reveals a fold similar to Bacillus anthracis lethal factor. The structure consists of an upper N-terminal domain and a lower helical C-terminal domain with respect to the active-site helix aalpha4, which harbors two of the three zinc-binding residues His142 and His146 and the catalytic base Glu143 in the typical metalloprotease motif (H142E143TAH146)	Clostridioides difficile

Crystallization (Comment)

Organism

crystal structures of Zmp1 in its unbound and peptide-bound forms. The structure analysis reveals a fold similar to Bacillus anthracis lethal factor. The structure consists of an upper N-terminal domain and a lower helical C-terminal domain with respect to the active-site helix aalpha4, which harbors two of the three zinc-binding residues His142 and His146 and the catalytic base Glu143 in the typical metalloprotease motif (H142E143TAH146)

Clostridioides difficile

Protein Variants

Protein Variants	Comment	Organism
E143A	18% of wild-type activity	Clostridioides difficile
E143A/Y178F	complete loss of activity	Clostridioides difficile
Y178F	41% of wild-type activity	Clostridioides difficile

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Clostridioides difficile	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	Q183R7	-	-
Clostridioides difficile 630	Q183R7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-EVNPPVPD + H2O	-	Clostridioides difficile	acetyl-EVNP + PVPD	-	?
acetyl-EVNPPVPD + H2O	-	Clostridioides difficile 630	acetyl-EVNP + PVPD	-	?
fibrinogen beta-chain + H2O	almost complete digestion within 1 h	Clostridioides difficile	?	-	?
fibrinogen beta-chain + H2O	almost complete digestion within 1 h	Clostridioides difficile 630	?	-	?
Fibronectin + H2O	-	Clostridioides difficile	?	poor substrate	?
Fibronectin + H2O	-	Clostridioides difficile 630	?	poor substrate	?

Synonyms

Synonyms	Comment	Organism
Zmp1	-	Clostridioides difficile