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Literature summary for 3.4.24.87 extracted from

  • South, K.; Luken, B.M.; Crawley, J.T.; Phillips, R.; Thomas, M.; Collins, R.F.; Deforche, L.; Vanhoorelbeke, K.; Lane, D.A.
    Conformational activation of ADAMTS13 (2014), Proc. Natl. Acad. Sci. USA, 111, 18578-18583.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.87

Crystallization (Commentary)

Crystallization (Comment) Organism
in electron microscopy, wild-type ADAMTS13 adopts a closed conformation, gain-of-function mutant R568K/F592Y/R660K/Y661F/Y665F shows a more open conformation Homo sapiens

Protein Variants

Protein Variants Comment Organism
R568K/F592Y/R660K/Y661F/Y665F gain-of-function ADAMTS13 spacer domain variant, about 2.5fold more active than wild-type ADAMTS13, but cannot be further activated by anti-CUB monoclonal antibody or von Willebrand factor D4CK and is unable to bind or to be inhibited by the CUB1-2 domains Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00086
-
 VWF115 mutant R568K/F592Y/R660K/Y661F/Y665F, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.00154
-
 VWF115 wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q76LX8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
VWF115 + H2O von Willebrand factor A2 domain fragment, residues 1554-1668 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.62
-
 VWF115 mutant R568K/F592Y/R660K/Y661F/Y665F, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.71
-
 VWF115 wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens

General Information

General Information Comment Organism
physiological function wild-type ADAMTS13 shows 2.5fold reduced activity compared with ADAMTS13 lacking its C-terminal tail or its CUB1-2 domains. Wild-type ADAMTS13 activity is enhanced about 2.5fold by preincubation with either an anti-CUB monoclonal antibody or von Willebrand factor D4CK, the natural binding partner for the CUB domains. The isolated CUB1-2 domains bind ADAMTS13 C-terminal tails, and also inhibit activity by up to 2.5fold Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
410
-
 VWF115 wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
830
-
 VWF115 mutant R568K/F592Y/R660K/Y661F/Y665F, pH not specified in the publication, temperature not specified in the publication Homo sapiens