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Literature summary for 3.4.24.87 extracted from

  • Hiura, H.; Matsui, T.; Matsumoto, M.; Hori, Y.; Isonishi, A.; Kato, S.; Iwamoto, T.; Mori, T.; Fujimura, Y.
    Proteolytic fragmentation and sugar chains of plasma ADAMTS13 purified by a conformation-dependent monoclonal antibody (2010), J. Biochem., 148, 403-411.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ ADAMTS13 activity is decreased in the presence of 0.9 mM Ni2+ Homo sapiens
Ni2+ ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Ni2+ Homo sapiens
Zn2+ ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Zn2+ Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ in the presence of 10 mM Ba2+, the activity is enhanced 1.1 to 1.4fold Homo sapiens
Ca2+ ADAMTS13 activity in the reaction buffer containing 0.9 mM Ca2+ without incubation is enhanced 2.7times compared to the value determined in the absence of Ca2+. The activity of ADAMTS13 in the presence of 10 mM Ca2+ is enhanced 3-5fold Homo sapiens
additional information the enzyme is not influenced in the presence of Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
x * 190000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
von Willebrand factor + H2O Homo sapiens ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
A10-agarose column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
unpurified blood plasma ADAMTS13 has a specific activity of 0.01 units/mg, purified blood plasma ADAMTS13 has a specific activity of 300 units/mg (25057fold purification) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
von Willebrand factor + H2O ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 190000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13
-
Homo sapiens
ADAMTS13
-
Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.1 8.8 the activity of purified pADAMTS13 is stable and relatively constant at pH ranges between pH 6.1 and 8.8 for 18 h storage at 4°C. However, ADAMTS13 activity is almost totally lost below pH 3.1 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens purified blood plasma ADAMTS13, isoelectric focusing 5.5 5.1

General Information

General Information Comment Organism
malfunction lack of blood plasma ADAMTS13 activity results in thrombotic thrombocytopenic purpura Homo sapiens