Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | ADAMTS13 activity is decreased in the presence of 0.9 mM Ni2+ | Homo sapiens | |
Ni2+ | ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Ni2+ | Homo sapiens | |
Zn2+ | ADAMTS13 activity is markedly decreased in the presence of 0.9 mM Zn2+ | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | in the presence of 10 mM Ba2+, the activity is enhanced 1.1 to 1.4fold | Homo sapiens | |
Ca2+ | ADAMTS13 activity in the reaction buffer containing 0.9 mM Ca2+ without incubation is enhanced 2.7times compared to the value determined in the absence of Ca2+. The activity of ADAMTS13 in the presence of 10 mM Ca2+ is enhanced 3-5fold | Homo sapiens | |
additional information | the enzyme is not influenced in the presence of Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
x * 190000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
von Willebrand factor + H2O | Homo sapiens | ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
A10-agarose column chromatography, HiTrap DEAE column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
unpurified blood plasma ADAMTS13 has a specific activity of 0.01 units/mg, purified blood plasma ADAMTS13 has a specific activity of 300 units/mg (25057fold purification) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
von Willebrand factor + H2O | ADAMTS13 specifically cleaves von Willbrand factor A2 domain between Tyr1605 and Met1606 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 190000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13 | - |
Homo sapiens |
ADAMTS13 | - |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8.8 | the activity of purified pADAMTS13 is stable and relatively constant at pH ranges between pH 6.1 and 8.8 for 18 h storage at 4°C. However, ADAMTS13 activity is almost totally lost below pH 3.1 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | purified blood plasma ADAMTS13, isoelectric focusing | 5.5 | 5.1 |
General Information | Comment | Organism |
---|---|---|
malfunction | lack of blood plasma ADAMTS13 activity results in thrombotic thrombocytopenic purpura | Homo sapiens |