Any feedback?
Literature summary for 3.4.24.87 extracted from
- ADAMTS13 bound to endothelial cells exhibits enhanced cleavage of von Willebrand factor (2009), J. Biol. Chem., 284, 30925-30932.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged ADAMTS13 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| heparin | inhibits ADAMTS13 activity through inhibition of enzyme binding to endothelial cell surfaces, overview | Homo sapiens |  |
| Trypsin | inhibits ADAMTS13 activity through inhibition of enzyme binding to endothelial cell surfaces, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | ADAMTS13 binds to endothelial cells in a specific, reversible, and time-dependent manner with a Kd of 58 nM. Binding requires the COOH-terminal thrombospondin type 1 repeats of the protease. Binding is inhibited in the presence of heparin and by trypsin treatment of the cells | Homo sapiens | 9986 | - |
| extracellular | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| von Willebrand factor + H2O | Homo sapiens | cleavage can also occur at the surface of endothelial cells. ADAMTS13 that is prebound to endothelial cells exhibits increased proteolysis of VWF as compared with ADAMTS13 present only in solution. Thus, cleavage of VWF occurs mainly at the endothelial cell surface | von Willebrand factor fragments | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged ADAMTS13 by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endothelial cell | at the surface of endothelial cells | Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
| umbilical vein endothelial cell | at the surface of endothelial cells | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| von Willebrand factor + H2O | - |
Homo sapiens | von Willebrand factor fragments | - |
? | |
| von Willebrand factor + H2O | cleavage can also occur at the surface of endothelial cells. ADAMTS13 that is prebound to endothelial cells exhibits increased proteolysis of VWF as compared with ADAMTS13 present only in solution. Thus, cleavage of VWF occurs mainly at the endothelial cell surface | Homo sapiens | von Willebrand factor fragments | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAMTS13 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | ADAMTS13 is a plasma metalloprotease that cleaves ultra-large von Willebrand factor multimers to generate less thrombogenic fragments | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
