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Literature summary for 3.4.24.87 extracted from

  • Raife, T.J.; Cao, W.; Atkinson, B.S.; Bedell, B.; Montgomery, R.R.; Lentz, S.R.; Johnson, G.F.; Zheng, X.L.
    Leukocyte proteases cleave von Willebrand factor at or near the ADAMTS13 cleavage site (2009), Blood, 114, 1666-1674.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.87

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of His-tagged ADAMTS13 in HEK-293 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
von Willebrand factor + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged ADAMTS13 from HEK-293 cells by anion exchange chromatography, Ni affinity chromatography, and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FRETSVWF73 peptide + H2O a von Willebrand factor-derived fluorescein-labeled peptide substrate Homo sapiens ?
-
 ?
von Willebrand factor + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ADAMTS13
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.43
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information under denaturing and fluid shear stress conditions multiple leukocyte proteases cleave von Willebrand factor predominantly in the central A2 domain. Activated neutrophils, but not normal neutrophils, retain von Willebrand factor-cleaving activity in the presence of plasma inhibitors, suggesting that leukocyte proteases may regulate von Willebrand factor function under physiologic conditions, kinetics and reaction products, overview Homo sapiens
physiological function importance of ADAMTS13 metalloprotease in von Willebrand factor regulation and association between severe deficiency of ADAMTS13 and thrombotic thrombocytopenic purpura. However, ADAMTS13 activity levels do not always correlate with the clinical course of TTP, suggesting that other proteases, e.g. elastase or proteinase 3 expressed on leukocyte surfaces, might be important in regulating von Willebrand factor Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
763.8
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens