Cloned (Comment) | Organism |
---|---|
gene Adamts13, DNA and amino acid sequence determination, construction of self-inactivated lentiviral vector, and expression of functional His-tagged ADAMTS13 cDNA in COS-7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | plasma ADAMTS13 proteolytic activity on average can be restored in Adamts13-/- mice to approximately 25% of wild-type level by autologous transplantation of hematopoietic progenitor cells transduced ex vivo with a self-inactivating lentiviral vector encoding a full-length murine Adamts13 and an enhanced GFP reporter gene | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Mus musculus | |
Ca2+ | activates | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
von Willebrand factor + H2O | Mus musculus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene Adamts13 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of ADAMTS13 antigen and proteolytic activity in murine plasma, overview | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | assay on the fluorogenic substrate FRETS-vWF73 | Mus musculus | ? | - |
? | |
von Willebrand factor + H2O | - |
Mus musculus | ? | - |
? | |
von Willebrand factor + H2O | substrate of human origin | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS13 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
additional information | genetic or acquired deficiency of plasma ADAMTS13 activity leads to a potentially fatal syndrome, thrombotic thrombocytopenic purpura, TTP | Mus musculus |