Literature summary for 3.4.24.87 extracted from

Akiyama, M.; Takeda, S.; Kokame, K.; Takagi, J.; Miyata, T.
Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13 (2009), Acta Crystallogr. Sect. F, 65, 739-742.

Search for more literature for 3.4.24.87

Cloned(Commentary)

Cloned (Comment)	Organism
stable expression of His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, in CHO Lec cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant His-tagged ADAMTS13 ancillary domains, residues 287-685, sitting-drop vapour-diffusion method, mixing of 100 nl protein solution, containing about 20 mg/ml protein, with an equal amount of reservoir solution, containing 26% w/v PEG 1500, 100 mM MES, pH 6.0, equilibration against 0.1 ml of reservoir solution, at 20°C, for 24 h, crystals are soaked in a solution containing 20% glycerol, 26% PEG 1500, 100 mM MES, pH 6.0, for cryoprotection, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, labeling with heavy-atom derivatives	Homo sapiens

Crystallization (Comment)

Organism

recombinant His-tagged ADAMTS13 ancillary domains, residues 287-685, sitting-drop vapour-diffusion method, mixing of 100 nl protein solution, containing about 20 mg/ml protein, with an equal amount of reservoir solution, containing 26% w/v PEG 1500, 100 mM MES, pH 6.0, equilibration against 0.1 ml of reservoir solution, at 20°C, for 24 h, crystals are soaked in a solution containing 20% glycerol, 26% PEG 1500, 100 mM MES, pH 6.0, for cryoprotection, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, labeling with heavy-atom derivatives

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	replacement of signal sequence and prosequence with the mouse Nid1 signal sequence dramatically increases the secretion of ADAMTS13-DTCS into the medium	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
von Willebrand factor + H2O	Homo sapiens	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q76LX8	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the ADAMTS13 ancillary domains, ADAMTS13-DTCS, contain four potential N-glycosylation sites	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, from CHO Lec cells by nickel affinity and cation exchange chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
plasma	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
von Willebrand factor + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE	Homo sapiens
More	ADAMTS13 is a multi-domain enzyme. In addition to the N-terminal metalloproteinase domain, the ancillary domains, including a disintegrin-like domain, a thrombospondin-1 type 1 repeat, a Cys-rich domain and a spacer domain, are required for von Willebrand factor recognition and cleavage	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ADAMTS13	-	Homo sapiens
More	ADAMTS13 is a reprolysin-type metalloproteinase belonging to the ADAMTS, i.e. a disintegrin and metalloproteinase with thrombospondin type 1 motif, family	Homo sapiens
von Willebrand factor-cleaving proteinase	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	ADAMTS13 specifically cleaves plasma von Willebrand factor and regulates platelet adhesion and aggregation	Homo sapiens