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Literature summary for 3.4.24.87 extracted from
- Zinc and calcium ions cooperatively modulate ADAMTS13 activity (2006), J. Biol. Chem., 281, 850-857.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | an increasing concentration of CoCl2 inhibits ADAMTS13 activity | Homo sapiens |  |
| MnSO4 | an increasing concentration of MnSO4 inhibits ADAMTS13 activity | Homo sapiens | |
| additional information | increasing concentrations of BaCl2 (up to 5 mM) have little effect on the activity of ADAMTS13 at pH 7.4, Mg(SO4) and Cu(SO4) have little effect on the activity of ADAMTS13 at pH 7.4 | Homo sapiens | |
| NiSO4 | an increasing concentration of NiSO4 inhibits ADAMTS13 activity | Homo sapiens | |
| Zn2+ | ADAMTS13 activity is undetectable at concentrations of zinc ions above 3 mM Zn2+ | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km: 0.0032 mM for FRETS-VWF73, a fluorescent synthetic peptide, corresponding to residues Asp1596-Arg1668 of von Willebrand factor domain A2 and containing the Tyr1605-Met1606 bond cleaved by ADAMTS13 | Homo sapiens | |
| 0.0032 | - |
FRETS-von Willebrand factor 73 | apparent value, pH 7.4 | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | barium ions stimulate ADAMTS13 activity in citrated plasma but not in citrate-free plasma, cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by barium ions | Homo sapiens | |
| Ca2+ | cooperative role for Ca2+ and Zn2+ in supporting ADAMTS13 activity | Homo sapiens | |
| Ca2+ | in citrate-anticoagulated plasma activity is enhanced about 3fold by calcium ions (up to 5 mM), cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by calcium ions | Homo sapiens | |
| Zn2+ | cooperative role for Ca2+ and Zn2+ in supporting ADAMTS13 activity | Homo sapiens | |
| Zn2+ | in citrate-anticoagulated plasma activity is enhanced about 2fold by zinc ions (1 mM), cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by zinc ions | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| von Willebrand factor + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FRETS-von Willebrand factor 73 + H2O | - |
Homo sapiens | ? | - |
? | |
| von Willebrand factor + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAMTS13 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat: 0.83 per min for FRETS-VWF73, a fluorescent synthetic peptide, corresponding to residues Asp1596-Arg1668 of von Willebrand factor domain A2 and containing the Tyr1605-Met1606 bond cleaved by ADAMTS13 | Homo sapiens |
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