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Literature summary for 3.4.24.87 extracted from

  • Perutelli, P.; Amato, S.; Molinari, A.C.
    Cleavage of von Willebrand factor by ADAMTS-13 in vitro: effect of temperature and barium ions on the proteolysis kinetics (2005), Blood Coagul. Fibrinolysis, 16, 607-611.
    View publication on PubMed

Application

Application Comment Organism
medicine description of a simple procedure to analyse the kinetics of von Willebrand factor proteolysis that is suitable for routine diagnostic use Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ complete proteolysis is observed at 37°C in the presence of BaCl2 while about 25% von Willebrand factor still binds to collagen when BaCl2 supplementation is omitted Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Source Tissue

Source Tissue Comment Organism Textmining
blood plasma platelet-poor Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
von Willebrand factor + H2O complete proteolysis is observed at 37°C in the presence of BaCl2 while about 25% von Willebrand factor still binds to collagen when BaCl2 supplementation is omitted. Proteolysis kinetics at 22°C and 4°C is slower but complete Homo sapiens 2 peptides of 140 kDa and 176 kDa
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