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Literature summary for 3.4.24.84 extracted from

  • Corrigan, D.P.; Kuszczak, D.; Rusinol, A.E.; Thewke, D.P.; Hrycyna, C.A.; Michaelis, S.; Sinensky, M.S.
    Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24 (2005), Biochem. J., 387, 129-138.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf-21 insect cells Homo sapiens
expression in CHO-K1 cells Homo sapiens
Zmpste24, fused N-terminally to a His6 tag, cloned into the (5')-NheI-(3')-BamHI site of the pcDNA3.1 construct using reverse transcriptase-PCR on HeLa RNA, when cloned into pBacpak8 Homo sapiens

Protein Variants

Protein Variants Comment Organism
L647R prelaminaAct mutant, cannot be cleaved by Zmpste24 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline Zn2+ chelator Homo sapiens
lovastatin abolishes the conversion of prelamin A into lamin A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
membrane
-
 Homo sapiens 16020
-
nuclear envelope
-
 Homo sapiens 5635
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-dependent active site Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 SDS-PAGE or immunoblot analyis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-bead-purified Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a-factor + H2O the enzyme has CAAX endopeptidase activity towards a-factor substrate Homo sapiens ?
-
 ?
N-(Ac)-Cys-(farnesyl)-Ser-Ile-Met + H2O the enzyme can process the prelaminA-specific CAAX sequence Homo sapiens ?
-
 ?
prelamin A + H2O cleavage is dependent on processing at the CAAX-box Homo sapiens lamin A
-
 ?

Synonyms

Synonyms Comment Organism
Zmpste24
-
 Homo sapiens