Cloned (Comment) | Organism |
---|---|
expressed in Sf-21 insect cells | Homo sapiens |
expression in CHO-K1 cells | Homo sapiens |
Zmpste24, fused N-terminally to a His6 tag, cloned into the (5')-NheI-(3')-BamHI site of the pcDNA3.1 construct using reverse transcriptase-PCR on HeLa RNA, when cloned into pBacpak8 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L647R | prelaminaAct mutant, cannot be cleaved by Zmpste24 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | Zn2+ chelator | Homo sapiens | |
lovastatin | abolishes the conversion of prelamin A into lamin A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
membrane | - |
Homo sapiens | 16020 | - |
nuclear envelope | - |
Homo sapiens | 5635 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+-dependent active site | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
SDS-PAGE or immunoblot analyis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-bead-purified | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a-factor + H2O | the enzyme has CAAX endopeptidase activity towards a-factor substrate | Homo sapiens | ? | - |
? | |
N-(Ac)-Cys-(farnesyl)-Ser-Ile-Met + H2O | the enzyme can process the prelaminA-specific CAAX sequence | Homo sapiens | ? | - |
? | |
prelamin A + H2O | cleavage is dependent on processing at the CAAX-box | Homo sapiens | lamin A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Zmpste24 | - |
Homo sapiens |