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Literature summary for 3.4.24.83 extracted from

  • Shaw, C.A.; Starnbach, M.N.
    Both CD4+ and CD8+ T cells respond to antigens fused to anthrax lethal toxin (2008), Infect. Immun., 76, 2603-2611.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
molecular biology results suggest that this toxin delivery system is capable of stimulating protective immune responses where effective immunization requires stimulation of both classes of T cells Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant anthrax lethal toxin proteins consisting a model CD4 T-cell epitope from chicken ovalbumin (Ova) fused to nontoxic lethal factor (LFn). The antigen tags are generated by annealing single-stranded DNA oligonucleotides to form double-stranded DNA Plasmids are transformed into Escherichia coli BL21 Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
additional information in order to examine the role of protective antigen a lethal factor (LFn) fusion protein bearing two epitopes from Ova, one restricted by MHC-II and one restricted by MHC-I is generated. This single LFn fusion protein is capable of stimulating both ovalbumin-specific CD4+ and ovalbumin-specific CD8+ T-cell responses in mice Bacillus anthracis
additional information it is investigated whether lethal factor (LFn) fusion proteins and protective antigen can also be used to deliver antigen to the MHC-II pathway for the stimulation of antigen-specific CD4+ T-cells. A CD4+ T-cell epitope from chicken ovalbumin is fused to nontoxic LFn and demonstrates that this recombinant protein induces an ovalbumin-specific CD4+ T-cell response both in vitro and in mice Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
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-
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Purification (Commentary)

Purification (Comment) Organism
the recombinant LFn fusion proteins contain a vector-encoded His6 tag at the amino terminus and are purified by Ni2+-affinity chromatography to greater than 95% purity as determined by Coomassie staining Bacillus anthracis

Synonyms

Synonyms Comment Organism
anthrax lethal toxin
-
 Bacillus anthracis
lethal factor lethal toxin is secreted by Bacillus anthracis as a bipartite toxin consisting of protective antigen (PA) and lethal factor (LF) Bacillus anthracis