Literature summary for 3.4.24.82 extracted from

Kashiwagi, M.; Enghild, J.J.; Gendron, C.; Hughes, C.; Caterson, B.; Itoh, Y.; Nagase, H.
Altered Proteolytic Activities of ADAMTS-4 Expressed by C-terminal Processing (2004), J. Biol. Chem., 279, 10109-10119.

Search for more literature for 3.4.24.82

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in 293-EBNA cells	Sus scrofa

Protein Variants

Protein Variants	Comment	Organism
E362A	inactive mutant enzyme with mutation of Glu to Ala at position 362	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by tissue inhibitor of metalloproteinases 1 or tissue inhibitor of metalloproteinases 2 at 100 nM	Sus scrofa
tissue inhibitor of metalloproteinases 3	20 nM, complete inhibition	Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	SDS-PAGE, small amount of ADAMTS4 found in porcupine articular cartilage without interleukin-1alpha treatment	Sus scrofa
40000	-	SDS-PAGE, small amount of ADAMTS4 found in porcupine articular cartilage without interleukin-1alpha treatment	Sus scrofa
46000	-	SDS-PAGE, primary form of ADAMTS4 in the interleukine-1alpha-treated porcupine articular cartilage	Sus scrofa
70000	-	SDS-PAGE, full-length enzyme	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
anti-FLAG affinity chromatography and S-200 Sephacryl gel filtration	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
chondrocyte	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	39.8 units/nmol protein, one unit of enzyme activity represents the release of 0.001 mg of glycosaminoglycan/min at 37°C at pH 7.5, wild type enzyme	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aggrecan + H2O	-	Sus scrofa	fragments of aggrecan	-	?
decorin + H2O	-	Sus scrofa	?	-	?
fibromodulin + H2O	-	Sus scrofa	?	-	?
transferrin + H2O	-	Sus scrofa	?	-	?

Synonyms

Synonyms	Comment	Organism
ADAMTS-4	-	Sus scrofa
aggrecanase 1	-	Sus scrofa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)