Literature summary for 3.4.24.82 extracted from

Tortorella, M.; Pratta, M.; Liu, R.Q.; Abbaszade, I.; Ross, H.; Burn, T.; Arner, E.
The thrombospondin motif of aggrecanase-1 (ADAMTS-4) is critical for aggrecan substrate recognition and cleavage (2000), J. Biol. Chem., 275, 25791-25797.

Search for more literature for 3.4.24.82

Cloned(Commentary)

Cloned (Comment)	Organism
expression of full length aggrecanase-1 and a C-terminal truncated form lacking the disintegrin and TSP motif in Drosophila S2 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
GGWGPWGPWGD	peptide representing the N-terminal region of the aggrecane TPS-1 motif containign the GAG binding motif, 0.017 mM, 50% inhibition	Homo sapiens
GGWGPWGPWGDCSRTCGGG	peptide containing both the GAG and CD36 binding motifs of aggrecan, 0.003 mM, 50% inhibition	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O75173	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant ADAMTS-4	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aggrecan + H2O	the thrombospondin type-1 motif located within the C-terminus of aggrecanase-1 is critical for substrate binding and cleavage	Homo sapiens	fragments of aggrecan	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000105	-	GGWGPWGPWGDCSRTCGGG	-	Homo sapiens

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Release 2023.1 (January 2023)