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Literature summary for 3.4.24.78 extracted from
- Partial characterization of an enzyme fraction with protease activity which converts the spore peptidoglycan hydrolase (SleC) precursor to an active enzyme during germination of Clostridium perfringens S40 spores and analysis of a gene cluster involved in the activity (2001), J. Bacteriol., 183, 3742-3751.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | 1 mM, 73% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens |  |
| additional information | not inhibited by E-64, pepstatin, bestatin, EDTA, antipain, APMSF, leupeptin, aprotinin and SSI | Clostridium perfringens | |
| p-chloromercuribenzenesulfonate | 1 mM, 39% inhibition, addition of 10 mM dithiothreitol restores activity to 100% | Clostridium perfringens | |
| PMSF | 1 mM, 68% inhibition after 1 h | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51442 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
| 52000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
| 55458 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
| 55585 | - |
x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
| 57000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
| 60000 | - |
x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Superose 12, hydroxyapatite, DEAE-5PW | Clostridium perfringens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed coat | coat of dormant spores | Clostridium perfringens | - |
| spore | - |
Clostridium perfringens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
139363 units, 1 unit is defined as a decrease in the OD600 of 0.1 per min | Clostridium perfringens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000 + x * 57000 + x * 60000, processed forms of preproteins, SDS-PAGE | Clostridium perfringens |
| ? | x * 51442 + x * 55458 + x * 55585, deduced from nucleotide sequence | Clostridium perfringens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
72 h, 80% loss of activity in the absence of CHAPS | Clostridium perfringens |
| 55 | - |
40 min at pH 7.0, no loss of activity in the presence of 0.1% CHAPS | Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.5 | - |
Clostridium perfringens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
at least 2 month at 4°C in the presence of 0.1% CHAPS, no loss of activity | Clostridium perfringens |
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