Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus simulans |
Crystallization (Comment) | Organism |
---|---|
lysostaphin SH3b domain in complex with a pentaglycine peptide, hanging drop vapor diffusion method | Staphylococcus simulans |
Protein Variants | Comment | Organism |
---|---|---|
N405A | the mutation does not affect lytic activity of the mature enzyme | Staphylococcus simulans |
N405A/Y472A | the mutations do not affect lytic activity of the mature enzyme | Staphylococcus simulans |
Y472A | the mutation does not affect lytic activity of the mature enzyme | Staphylococcus simulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | P10547 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography and Superdex 75gel filtration | Staphylococcus simulans |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Staphylococcus aureus mutant strain femAB cell wall + H2O | - |
Staphylococcus simulans | ? | - |
? | |
Staphylococcus aureus strain TF5311 cell wall + H2O | - |
Staphylococcus simulans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SH3b | cell wall binding domain | Staphylococcus simulans |