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Literature summary for 3.4.24.75 extracted from

  • Sabala, I.; Jagielska, E.; Bardelang, P.; Czapinska, H.; Dahms, S.; Sharpe, J.; James, R.; Than, M.; Thomas, N.; Bochtler, M.
    Crystal structure of the antimicrobial peptidase lysostaphin from Staphylococcus simulans (2014), FEBS J., 281, 4112-4122.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus simulans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES/NaOH pH 6.5 and 1.6 M magnesium sulfate, 2 mM tetraglycine phosphinic acid, or 0.1 M Tris (base), bicine pH 8.5, 0.1 M amino acids [L-Na-glutamate, L-alanine (racemic), glycine, L-lysine (racemic), L-serine (racemic)], 30% (v/v) PEG 8000, or 0.05 M potassium dihydrogen phosphate and 20% (w/v) PEG 8000 Staphylococcus simulans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains one zinc ion Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus simulans