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Literature summary for 3.4.24.75 extracted from

  • Lu, H.; Gu, M.; Huang, Q.; Huang, J.; Lu, W.; Lu, H.; Huang, Q.
    Hydrogen/deuterium exchange mass spectrometry and site-directed disulfide cross-linking suggest an important dynamic interface between the two lysostaphin domains (2013), Antimicrob. Agents Chemother., 57, 1872-1881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus simulans

Protein Variants

Protein Variants Comment Organism
D339C the mutant shows about 37% of wild type bacteriolytic activity Staphylococcus simulans
K332C the mutant shows about 82% of wild type bacteriolytic activity Staphylococcus simulans
K332C/T464C the mutant retains around 25% of the bacteriolytic activity of the wild type protein Staphylococcus simulans
K336C the mutant shows about 90% of wild type bacteriolytic activity Staphylococcus simulans
L258C the mutant shows about 35% of wild type bacteriolytic activity Staphylococcus simulans
N260C the mutant shows about 80% of wild type bacteriolytic activity Staphylococcus simulans
Q256C the mutant shows about 85% of wild type bacteriolytic activity Staphylococcus simulans
R470C the mutant shows about 50% of wild type bacteriolytic activity Staphylococcus simulans
T464C the mutant shows about 85% of wild type bacteriolytic activity Staphylococcus simulans
T477C the mutant shows wild type bacteriolytic activity Staphylococcus simulans
T482C the mutant shows wild type bacteriolytic activity Staphylococcus simulans
V487C the mutant shows wild type bacteriolytic activity Staphylococcus simulans

Inhibitors

Inhibitors Comment Organism Structure
additional information the bacteriolytic activity of the enzyme is reduced to about 60% in the presence of 150 mM NaCl which causes contractions of bacterial peptidoglycan substrate Staphylococcus simulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidoglycan + H2O Staphylococcus simulans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans P10547
-
-

Purification (Commentary)

Purification (Comment) Organism
Capto MMC column chromatography, and Sephadex G-50 gel filtration Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan + H2O
-
 Staphylococcus simulans ?
-
 ?