Application | Comment | Organism |
---|---|---|
medicine | lysostaphin is an antistaphylococcal agent. The enzyme is efficacious in the treatment of bovine mastitis caused by staphylococci | Staphylococcus simulans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Staphylococcus simulans |
General Stability | Organism |
---|---|
lysostaphin is relatively stable when conjugated with polyethylene glycol and it maintains its activity in human serum | Staphylococcus simulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Staphylococcus simulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, calculated from amino acid sequence | Staphylococcus simulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus simulans | lysostaphin exhibits a high degree of antistaphylococcal bacteriolytic activity, being inactive against bacteria of all other genera. The cell-wall degrading activity of lysostaphin is primarily due to a glycylglycine endopeptidase activity. Staphylococcus simulans biovar staphylolyticus peptidoglycan is resistant to the hydrolytic activity of lysostaphin, since the cells produce a resistance factor that causes the incorporation of Ser residues into the third and fifth positions of the cell wall cross bridges | ? | - |
? | |
Peptidoglycan + H2O | Staphylococcus simulans | cleaves Gly-Gly bonds in peptidoglycans | Fragments of peptidoglycan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | P10547 | Staphylococcus simulans biovar staphylolyticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lysostaphin exhibits a high degree of antistaphylococcal bacteriolytic activity, being inactive against bacteria of all other genera. The cell-wall degrading activity of lysostaphin is primarily due to a glycylglycine endopeptidase activity. Staphylococcus simulans biovar staphylolyticus peptidoglycan is resistant to the hydrolytic activity of lysostaphin, since the cells produce a resistance factor that causes the incorporation of Ser residues into the third and fifth positions of the cell wall cross bridges | Staphylococcus simulans | ? | - |
? | |
Peptidoglycan + H2O | cleaves Gly-Gly bonds in peptidoglycans | Staphylococcus simulans | Fragments of peptidoglycan | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, calculated from amino acid sequence | Staphylococcus simulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Staphylococcus simulans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus simulans | calculated from amino acid sequence | - |
9.5 |