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Literature summary for 3.4.24.74 extracted from

  • Goulas, T.; Arolas, J.L.; Gomis-Rueth, F.X.
    Structure, function and latency regulation of a bacterial enterotoxin potentially derived from a mammalian adamalysin/ADAM xenolog (2011), Proc. Natl. Acad. Sci. USA, 108, 1856-1861.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami-2 (DE3) cells Bacteroides fragilis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM sodium citrate dihydrate, 20% (w/v) PEG 3000, pH 5.5 Bacteroides fragilis

Protein Variants

Protein Variants Comment Organism
E349A residual activity against fibrinogen and fluorophore-labeled casein Bacteroides fragilis

Inhibitors

Inhibitors Comment Organism Structure
CT1746
-
 Bacteroides fragilis
additional information activity is completely abolished by standard zinc-chelating agents Bacteroides fragilis
Zn2+ activity is significantly impaired by excess zinc Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, SDS-PAGE Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
E-cadherin + H2O Bacteroides fragilis in vivo substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis O86049
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography Bacteroides fragilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-Asp-Tyr-Val-Ala-Ser-Glu-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
Abz-Lys-Asp-Glu-Ser-Tyr-Arg-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
Abz-Thr-Val-Leu-Glu-Arg-Ser-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
Abz-Tyr-Gly-Lys-Arg-Val-Phe-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
Abz-Val-Lys-Phe-Tyr-Asp-Ile-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
actin + H2O
-
 Bacteroides fragilis ?
-
 ?
azocasein + H2O
-
 Bacteroides fragilis ?
-
 ?
azocollagen + H2O
-
 Bacteroides fragilis ?
-
 ?
BODIPY FL-casein + H2O
-
 Bacteroides fragilis ?
-
 ?
casein + H2O
-
 Bacteroides fragilis ?
-
 ?
E-cadherin + H2O in vivo substrate Bacteroides fragilis ?
-
 ?
Fibrinogen + H2O
-
 Bacteroides fragilis ?
-
 ?
Fibronectin + H2O
-
 Bacteroides fragilis ?
-
 ?
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Bacteroides fragilis ?
-
 ?
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
-
 Bacteroides fragilis ?
-
 ?
additional information isozyme fargilysin-3 does not cleave azoalbumin Bacteroides fragilis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, SDS-PAGE Bacteroides fragilis

Synonyms

Synonyms Comment Organism
Bacteroides fragilis toxin
-
 Bacteroides fragilis
BFT-3 isozyme Bacteroides fragilis
fragilysin-3 isozyme Bacteroides fragilis