Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 36.2% residual activity at 5 mM | Bacillus amyloliquefaciens | |
Co2+ | 48.3% residual activity at 5 mM | Bacillus amyloliquefaciens | |
Cu2+ | 50% residual activity at 5 mM | Bacillus amyloliquefaciens | |
EDTA | 10.4% residual activity at 5 mM | Bacillus amyloliquefaciens | |
Fe2+ | 6.82% residual activity at 5 mM | Bacillus amyloliquefaciens | |
K+ | 98.6% residual activity at 5 mM | Bacillus amyloliquefaciens | |
Mg2+ | 96.8% residual activity at 5 mM | Bacillus amyloliquefaciens | |
pepstatin A | 48.2% residual activity at 5 mM | Bacillus amyloliquefaciens | |
phenylmethylsulfonyl fluoride | 8.48% residual activity at 5 mM | Bacillus amyloliquefaciens | |
Zn2+ | 52.6% residual activity at 5 mM | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 110% activity at 5 mM | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens UFPEDA 485 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrinogen + H2O | the highest fibrinolytic activity and a degradation of blood clot in vitro of 62% are obtained in a medium with 2% (w/v) soybean flour and 1% (w/v) D-glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37°C | Bacillus amyloliquefaciens | fibrin + ? | - |
? | |
fibrinogen + H2O | the highest fibrinolytic activity and a degradation of blood clot in vitro of 62% are obtained in a medium with 2% (w/v) soybean flour and 1% (w/v) D-glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37°C | Bacillus amyloliquefaciens UFPEDA 485 | fibrin + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fibrinolytic protease | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
after 12 h of incubation, the enzyme remains stable at 4, 25 and 37°C, maintaining activities of 96.04, 95.88, and 98.26%, respectively, and maintains 58.84% of its activity at 40°C | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
after incubation for 12 h, the enzyme retains 82.89% of its activity at pH 7.0, 66.03% at pH 8.0, and 50% at pH 6.0 and 9.0 after 8 h of incubation, respectively | Bacillus amyloliquefaciens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
at pH 7.0 and 37°C | Bacillus amyloliquefaciens | Cu2+ |