Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 88.8% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
Co2+ | 60.8% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
Cu2+ | 34.2% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
EDTA | 39.9% residual activity at 10 mM | Streptomyces sp. DPUA 1576 | |
Fe2+ | 95.3% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
Fe3+ | 95.4% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
Mg2+ | 94.4% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
additional information | not inhibited by iodoacetic acid | Streptomyces sp. DPUA 1576 | |
pepstatin A | 14.6% residual activity at 1 mM | Streptomyces sp. DPUA 1576 | |
phenylmethylsulfonyl fluoride | 25.4% residual activity at 50 mM | Streptomyces sp. DPUA 1576 | |
Zn2+ | 81.4% residual activity at 1 mM | Streptomyces sp. DPUA 1576 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Mn2+ | Streptomyces sp. DPUA 1576 | |
Na+ | the activity of protease is slightly enhanced (to 106%) by the presence of Na+ ions | Streptomyces sp. DPUA 1576 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. DPUA 1576 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Streptomyces sp. DPUA 1576 | ? | - |
? | |
Fibrin + H2O | - |
Streptomyces sp. DPUA 1576 | ? | - |
? | |
fibrinogen + H2O | - |
Streptomyces sp. DPUA 1576 | fibrin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Streptomyces sp. DPUA 1576 |
Synonyms | Comment | Organism |
---|---|---|
fibrinolytic enzyme | - |
Streptomyces sp. DPUA 1576 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
highest fibrinolytic activity | Streptomyces sp. DPUA 1576 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | highest protease activity between 35 and 55°C. The relative activities at 25°C and 75°C are 59% and 49%, respectively | Streptomyces sp. DPUA 1576 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | the enzyme is stable during 120 min at 25 and 35°C and shows 98, 100, and 59% residual activity at 25, 35 and 45°C, respectively. The enzyme in the crude extract loses its activity after 60 min between 55 and 75°C | Streptomyces sp. DPUA 1576 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimum pH for the fibrinolytic activity | Streptomyces sp. DPUA 1576 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | the enzyme activity decreases rapidly at levels below pH 7.0 and above pH 8.0 | Streptomyces sp. DPUA 1576 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9.2 | protease and fibrinolytic activities are stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively | Streptomyces sp. DPUA 1576 |