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Literature summary for 3.4.24.72 extracted from
- Enhanced production of fibrinolytic protease from Bacillus cereus NS-2 using cotton seed cake as nitrogen source (2013), Biocatal. Agricult. Biotechnol., 2, 204-209.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 21.6% inhibition at 10 mM | Bacillus cereus |  |
| Hg2+ | 63% inhibition at 10 mM | Bacillus cereus | |
| K+ | 30.9% inhibition at 10 mM | Bacillus cereus | |
| Pb2+ | 85.5% inhibition at 10 mM | Bacillus cereus | |
| SDS | 29% residual activity at 0.01% (w/v) | Bacillus cereus | |
| Triton X-100 | 87% residual activity at 0.01% (v/v) | Bacillus cereus | |
| Tween 20 | 80% residual activity at 0.01% (v/v) | Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 44.4% increase of activity at 10 mM | Bacillus cereus | |
| Cu2+ | 34.2% increase of activity at 10 mM | Bacillus cereus | |
| Fe2+ | 76.7% increase of activity at 10 mM | Bacillus cereus | |
| Mg2+ | 29% increase of activity at 10 mM | Bacillus cereus | |
| Mn2+ | 38% increase of activity at 10 mM | Bacillus cereus | |
| Zn2+ | 37.9% increase of activity at 10 mM | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrin + H2O | Bacillus cereus | - |
? | - |
? | |
| Fibrin + H2O | Bacillus cereus NS-2 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus NS-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrin + H2O | - |
Bacillus cereus | ? | - |
? | |
| Fibrin + H2O | - |
Bacillus cereus NS-2 | ? | - |
? | |
| Gelatin + H2O | - |
Bacillus cereus | ? | - |
? | |
| Gelatin + H2O | - |
Bacillus cereus NS-2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fibrinolytic protease | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | fibrinolytic protease loses about 40-60% of its activity after 1 h incubation at 20-80°C | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 12 | pre-incubation of fibrinolytic protease for 1 h at pH 4.0-12.0 causes substantial activity loss | Bacillus cereus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus cereus | cotton cake as nitrogen source enhances fibrinolytic protease production substantially (71%) as compared to control | up |
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