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Literature summary for 3.4.24.72 extracted from

  • Fang, H.M.; Zhao, L.; Lu, P.; Chen, S.J.; Bao, Z.X.; Qin, Y.F.; Zhu, Z.Y.; Zhao, J.M.; Mai, J.; Zhang, S.T.
    Modular design, expression and characterization of novel bifunctional mutants of fibrolase with combined platelet aggregation-inhibition and fibrinolytic activity (2011), Protein J., 30, 247-252.
    View publication on PubMed
Search for more literature for 3.4.24.72

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme does not require plasminogen or any other bloodborne intermediate for activity Agkistrodon contortrix contortrix

Cloned(Commentary)

Cloned (Comment) Organism
chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells Agkistrodon contortrix contortrix

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains one zinc ion in the active center Agkistrodon contortrix contortrix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 23000, SDS-PAGE Agkistrodon contortrix contortrix

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrinogen + H2O Agkistrodon contortrix contortrix the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 ?
-
 ?
additional information Agkistrodon contortrix contortrix chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agkistrodon contortrix contortrix P28891
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q-Sepharose column chromatography, gel filtration Agkistrodon contortrix contortrix

Renatured (Commentary)

Renatured (Comment) Organism
using 50 mM Tris-HCl, 0.5 M L-arginine, 1 M NaCl, 0.5 mM ZnCl2, 1 mM oxidized glutathione, 2 mM reduced glutathione, and 2 M urea at pH 7.0. After the protein is added, the pH is adjusted to 7.5, and the protein is left to refold at 4°C for approximately 24 h Agkistrodon contortrix contortrix

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Agkistrodon contortrix contortrix
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibrinogen + H2O the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 Agkistrodon contortrix contortrix ?
-
 ?
additional information chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase Agkistrodon contortrix contortrix ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Agkistrodon contortrix contortrix