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Literature summary for 3.4.24.72 extracted from

  • Swenson, S.; Toombs, C.F.; Pena, L.; Johansson, J.; Markland, F.S.
    alpha-Fibrinogenases (2004), Curr. Drug Targets Cardiovasc. Haematol. Disord., 4, 417-435.
    View publication on PubMed
Search for more literature for 3.4.24.72

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete and rapid inhibition Agkistrodon contortrix
alpha2-Macroglobulin
-
 Agkistrodon contortrix
EDTA complete and rapid inhibition Agkistrodon contortrix
Tetraethylenepentamine complete and rapid inhibition Agkistrodon contortrix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000242
-
 low molecular weight kininogen
-
 Agkistrodon contortrix
0.00000113
-
 His-Thr-Glu-Phe-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000138
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val
-
 Agkistrodon contortrix
0.00000164
-
 His-Thr-Glu-Glu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000247
-
 His-Thr-Glu-Lys-Phe-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000338
-
 His-Thr-Glu-Arg-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000391
-
 His-Thr-Glu-Pro-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000415
-
 His-Thr-Glu-His-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000528
-
 His-Thr-Glu-Leu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000579
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000739
-
 His-Thr-Glu-Ala-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000832
-
 His-Thr-Glu-Ser-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.00000937
-
 His-Thr-Glu-Asn-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.0000654
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
0.000579
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22890
-
 calculation from amino acid sequence Agkistrodon contortrix
60000
-
 polyethylene-glycosylated enzyme Agkistrodon contortrix

Organism

Organism UniProt Comment Textmining
Agkistrodon contortrix
-
 subspecies Agkistrodon contortrix contortrix
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein adduction of polyethylene glycol to reduce the rate of clearance from the circulation, and coupling to an Arg-Gly-Asp (RGD) like peptide imparting inhibitory activity on platelet aggregation and thrombus formation while maintaining full fibrinolytic activity Agkistrodon contortrix

Purification (Commentary)

Purification (Comment) Organism
high performance liquid chromatography, hydroxyapatite high performance liquid chromatography and cation exchange high performance liquid chromatography Agkistrodon contortrix

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Agkistrodon contortrix
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
 Agkistrodon contortrix Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
-
 ir
azocasein + H2O
-
 Agkistrodon contortrix ?
-
 ?
Fibrin + H2O
-
 Agkistrodon contortrix ?
-
 ?
Fibrinogen + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Ala-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Arg-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Asn-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Glu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-His-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Leu-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Ala-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Arg-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Asn-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Glu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-His-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Phe-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Pro-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Lys-Ser-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Phe-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Pro-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
His-Thr-Glu-Ser-Leu-Val-Thr-Ser + H2O
-
 Agkistrodon contortrix ?
-
 ?
Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val + H2O
-
 Agkistrodon contortrix ?
-
 ?
low molecular weight kininogen + H2O
-
 Agkistrodon contortrix kallidin + ?
-
 ?

Synonyms

Synonyms Comment Organism
fibrinolytic protease
-
 Agkistrodon contortrix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
0.0086
-
 low molecular weight kininogen
-
 Agkistrodon contortrix
0.09
-
 Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val
-
 Agkistrodon contortrix
0.16
-
 His-Thr-Glu-Leu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.2
-
 His-Thr-Glu-Lys-Phe-Val-Thr-Ser
-
 Agkistrodon contortrix
0.45
-
 His-Thr-Glu-Phe-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
0.46
-
 His-Thr-Glu-Glu-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
1.59
-
 His-Thr-Glu-Pro-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
1.97
-
 His-Thr-Glu-Arg-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
2.23
-
 His-Thr-Glu-Lys-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
3.98
-
 His-Thr-Glu-Ser-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
5
-
 His-Thr-Glu-Ala-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
5.19
-
 His-Thr-Glu-His-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix
6.03
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Agkistrodon contortrix
65.7
-
 His-Thr-Glu-Asn-Leu-Val-Thr-Ser
-
 Agkistrodon contortrix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Agkistrodon contortrix

pI Value

Organism Comment pI Value Maximum pI Value
Agkistrodon contortrix
-
-
 6.8