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Literature summary for 3.4.24.71 extracted from

  • Xu, D.; Emoto, N.; Giaid, A.; Slaughter, C.; Kaw, S.; deWit, D.; Yanagisawa, M.
    ECE-1: a membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1 (1994), Cell, 78, 473-485.
    View publication on PubMed
Search for more literature for 3.4.24.71

Cloned(Commentary)

Cloned (Comment) Organism
bovine Bos taurus
expressed in Chinese hamster ovary CHO-K1 cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Bos taurus
EDTA 0.03 mM, Zn2+ restores, Cu2+, Co2+, Mn2+ or Fe2+ partially restores, not Ca2+ or Mg2+ Bos taurus
FR901533 specific ECE-1 inhibitor, in vitro not in vivo Bos taurus
additional information leupeptin, chymostatin, pepstatin A, E-64, p-chloromercuriphenylsulfonic acid, N-ethylmaleimide or 4-amidinophenylmethyl sulfonylfluoride; no inhibition by thiorphan, captopril Bos taurus
phosphoramidon 0.1 mM, in vivo and in vitro Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85610
-
 bovine, deduced from nucleotide sequence Bos taurus
126000
-
 1 * 126000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-
endothelium vascular Bos taurus
-
additional information tissue distribution of mRNA Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Human big endothelin 1 + H2O best substrate Bos taurus Endothelin 1 + big endothelin 1(21-38)
-
 ?
Human big endothelin 1 + H2O cleavage site: Trp21-Val22 Bos taurus Endothelin 1 + big endothelin 1(21-38)
-
 ?
Human big endothelin 2 + H2O hydrolysis at about 40% the rate of big endothelin1 Bos taurus ?
-
 ?
Human big endothelin 3 + H2O hydrolysis at about 30% the rate of big endothelin1 Bos taurus ?
-
 ?
Human big endothelin 3 + H2O big endothelin 3(1-41)amide Bos taurus ?
-
 ?
additional information no substrate is the big endothelin 1(1-31) fragment Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 126000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7.8 about half-maximal activity at pH 5.8 and 7.8 Bos taurus